RRC ID 41342
Author Takenaka K, Fujiyama Y, Andoh A, Sasaki T, Amakata Y, Matsubara H, Hodohara K, Bamba T.
Title Prevention of murine acute graft-versus-host disease by staphylococcal enterotoxin B treatment.
Journal Clin Exp Immunol
Abstract Retroviral superantigens such as minor lymphocyte stimulating (Mls) antigen play an important role in the pathogenesis of acute graft-versus-host disease (GVHD). However, it remains unclear how exogenous bacterial superantigens modulate acute GVHD. In this study, we tested the effects of staphylococcal enterotoxin B (SEB) on the development of acute GVHD in a model involving the systemic transfer of parental C57Bl/6 spleen cells into BDF1 mice. SEB treatment suppressed the expansion of donor-derived T cells and blocked the decrease in the number of host cells. Impaired haematopoiesis was actually rescued by treatment with SEB. In SEB-treated mice, both spontaneous proliferation and IL-2 production in T cells were suppressed on day 2 after parental cell infusion. On day 21, the number of donor-derived CD4+ Vbeta8+ T cells markedly decreased in the spleen of SEB-treated mice. Donor-derived CD4+ T cells failed to proliferate in response to host alloantigens, and both donor- and host-derived T cells were unable to produce IL-2 in response to concanavalin A stimulation, suggesting that SEB treatment induced a general immunosuppressive state. Our results indicate that SEB treatment prevents the development of acute GVHD by leading to unresponsiveness of donor-derived T cells against host alloantigens in a Vbeta-restricted and unrestricted manner.
Volume 123(1)
Pages 155-61
Published 2001-1
DOI 10.1046/j.1365-2249.2001.01426.x
PII cei1426
PMID 11168013
PMC PMC1905963
MeSH Acute Disease Animals Cell Division / immunology Cells, Cultured Cytotoxicity Tests, Immunologic Disease Models, Animal Enterotoxins / administration & dosage Enterotoxins / therapeutic use* Female Graft vs Host Disease / blood Graft vs Host Disease / immunology* Graft vs Host Disease / pathology Graft vs Host Disease / prevention & control* Hematopoiesis / immunology Immunosuppressive Agents / administration & dosage Injections, Intraperitoneal Lymphocyte Activation / immunology Lymphocyte Depletion Mice Mice, Inbred C57BL Mice, Inbred DBA Receptors, Antigen, T-Cell, alpha-beta / antagonists & inhibitors Receptors, Antigen, T-Cell, alpha-beta / biosynthesis Staphylococcus aureus / immunology* T-Lymphocyte Subsets / immunology T-Lymphocyte Subsets / metabolism T-Lymphocyte Subsets / pathology T-Lymphocytes, Cytotoxic / immunology
IF 3.711
Times Cited 2
Human and Animal Cells