| RRC ID |
41342
|
| 著者 |
Takenaka K, Fujiyama Y, Andoh A, Sasaki T, Amakata Y, Matsubara H, Hodohara K, Bamba T.
|
| タイトル |
Prevention of murine acute graft-versus-host disease by staphylococcal enterotoxin B treatment.
|
| ジャーナル |
Clin Exp Immunol
|
| Abstract |
Retroviral superantigens such as minor lymphocyte stimulating (Mls) antigen play an important role in the pathogenesis of acute graft-versus-host disease (GVHD). However, it remains unclear how exogenous bacterial superantigens modulate acute GVHD. In this study, we tested the effects of staphylococcal enterotoxin B (SEB) on the development of acute GVHD in a model involving the systemic transfer of parental C57Bl/6 spleen cells into BDF1 mice. SEB treatment suppressed the expansion of donor-derived T cells and blocked the decrease in the number of host cells. Impaired haematopoiesis was actually rescued by treatment with SEB. In SEB-treated mice, both spontaneous proliferation and IL-2 production in T cells were suppressed on day 2 after parental cell infusion. On day 21, the number of donor-derived CD4+ Vbeta8+ T cells markedly decreased in the spleen of SEB-treated mice. Donor-derived CD4+ T cells failed to proliferate in response to host alloantigens, and both donor- and host-derived T cells were unable to produce IL-2 in response to concanavalin A stimulation, suggesting that SEB treatment induced a general immunosuppressive state. Our results indicate that SEB treatment prevents the development of acute GVHD by leading to unresponsiveness of donor-derived T cells against host alloantigens in a Vbeta-restricted and unrestricted manner.
|
| 巻・号 |
123(1)
|
| ページ |
155-61
|
| 公開日 |
2001-1-1
|
| DOI |
10.1046/j.1365-2249.2001.01426.x
|
| PII |
cei1426
|
| PMID |
11168013
|
| PMC |
PMC1905963
|
| MeSH |
Acute Disease
Animals
Cell Division / immunology
Cells, Cultured
Cytotoxicity Tests, Immunologic
Disease Models, Animal
Enterotoxins / administration & dosage
Enterotoxins / therapeutic use*
Female
Graft vs Host Disease / blood
Graft vs Host Disease / immunology*
Graft vs Host Disease / pathology
Graft vs Host Disease / prevention & control*
Hematopoiesis / immunology
Immunosuppressive Agents / administration & dosage
Injections, Intraperitoneal
Lymphocyte Activation / immunology
Lymphocyte Depletion
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Receptors, Antigen, T-Cell, alpha-beta / antagonists & inhibitors
Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
Staphylococcus aureus / immunology*
T-Lymphocyte Subsets / immunology
T-Lymphocyte Subsets / metabolism
T-Lymphocyte Subsets / pathology
T-Lymphocytes, Cytotoxic / immunology
|
| IF |
3.532
|
| 引用数 |
2
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
