RRC ID 41346
著者 Okamoto T, Niu R, Matsuo K, Furuhashi M, Ohsawa M, Mizutani S, Suzuki H.
タイトル Human chorionic gonadotropin beta-core fragment is directly produced by cancer cells.
ジャーナル Life Sci
Abstract The present study was undertaken to investigate whether hCG beta-core fragment (hCGbeta cf) was directly produced by cancer cells. Fifteen cell lines, including four choriocarcinoma and five ovarian cancer cell lines, were tested, and immunoreactivity of hCGbeta cf was present in the culture media of five of the cell lines. It was also present in the culture media of Chinese hamster ovary (CHO) cells transfected with hCGbeta gene. In addition to hCGbeta cf, gel chromatography and Western blot analysis of the culture media showed the presence of an hCGbeta cf immunoreactive material with a molecular weight of approximately 40 kDa. In an in vivo study, hCGbeta cf immunoreactivity was detected in the sera of the mice transplanted with NaUCC-3 choriocarcinoma cells, although the ratios of hCGbeta cf/hCG and hCGbeta cf/free hCGbeta were lower than those in the culture medium. Incubation experiments of purified hCGbeta cf in the serum showed no substantial decrease in its values, ruling out the possibility that formation of a macromolecule with serum components may mask hCGbeta cf immunoreactivity in the serum. Taken together, these results indicate that hCGbeta cf immunoreactive materials are directly produced by cancer cells and hCGbeta cf is not a urinary metabolite of hCG or hCGbeta alone. Also, reduced levels of hCGbeta cf in the serum compared with that of intact hCG or free hCGbeta are likely due to its short half-life.
巻・号 68(8)
ページ 861-72
公開日 2001-1-12
DOI 10.1016/s0024-3205(00)00986-3
PII S0024320500009863
PMID 11213356
MeSH Animals Biomarkers, Tumor / biosynthesis* Biomarkers, Tumor / blood Biomarkers, Tumor / immunology Blotting, Western CHO Cells / metabolism Carcinoma / metabolism Choriocarcinoma / blood Choriocarcinoma / metabolism Chorionic Gonadotropin / biosynthesis Chorionic Gonadotropin / immunology Chorionic Gonadotropin, beta Subunit, Human / biosynthesis* Chorionic Gonadotropin, beta Subunit, Human / blood Chorionic Gonadotropin, beta Subunit, Human / genetics Chorionic Gonadotropin, beta Subunit, Human / immunology Chromatography, Gel Cricetinae Culture Media Female Humans Immunoenzyme Techniques Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation Neoplasms / blood Neoplasms / metabolism* Ovarian Neoplasms / metabolism Peptide Fragments / biosynthesis* Peptide Fragments / blood Peptide Fragments / immunology Sarcoma / metabolism Transfection Transplantation, Heterologous Tumor Cells, Cultured
IF 3.647
引用数 10
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 Saos-2(RCB0428)