Reference - Detail
| RRC ID | 41356 |
|---|---|
| Author | Ohgami N, Nagai R, Miyazaki A, Ikemoto M, Arai H, Horiuchi S, Nakayama H. |
| Title | Scavenger receptor class B type I-mediated reverse cholesterol transport is inhibited by advanced glycation end products. |
| Journal | J Biol Chem |
| Abstract |
Cellular interactions of advanced glycation end products (AGE) are mediated by AGE receptors. We demonstrated previously that class A scavenger receptor types I and II (SR-A) and CD36, a member of class B scavenger receptor family, serve as the AGE receptors. In this study, we investigated whether scavenger receptor class B type I (SR-BI), another receptor belonging to class B scavenger receptor family, was also an AGE receptor. We used Chinese hamster ovary (CHO) cells overexpressed hamster SR-BI (CHO-SR-BI cells). (125)I-AGE-bovine serum albumin (AGE-BSA) was endocytosed in a dose-dependent fashion and underwent lysosomal degradation by CHO-SR-BI cells. (125)I-AGE-BSA exhibited saturable binding to CHO-SR-BI cells (K(d) = 8.3 microg/ml). Endocytic uptake of (125)I-AGE-BSA by CHO-SR-BI cells was completely inhibited by oxidized low density lipoprotein (LDL) and acetylated LDL, whereas LDL exerted only a weak inhibitory effect (<20%). Cross-competition experiments showed that AGE-BSA had no effect on HDL binding to these cells and vice versa. Interestingly, however, SR-BI-mediated selective uptake of HDL-CE was completely inhibited by AGE-BSA in a dose-dependent manner (IC(50) <10 microg/ml). Furthermore, AGE-BSA partially inhibited (by <30%) the selective uptake of HDL-CE in human hepatocarcinoma HepG2 cells (IC(50) <30 microg/ml). In addition, [(3)H]cholesterol efflux from CHO-SR-BI cells to HDL was significantly inhibited by AGE-BSA in a dose-dependent manner (IC(50) <30 microg/ml). Our results indicate that AGE proteins, as ligands for SR-BI, effectively inhibit both SR-BI-mediated selective uptake of HDL-CE and cholesterol efflux from peripheral cells to HDL, suggesting that AGE proteins might modulate SR-BI-mediated cholesterol metabolism in vivo. |
| Volume | 276(16) |
| Pages | 13348-55 |
| Published | 2001-4-20 |
| DOI | 10.1074/jbc.M011613200 |
| PII | S0021-9258(19)34460-6 |
| PMID | 11278947 |
| MeSH | Animals Binding, Competitive CD36 Antigens / genetics CD36 Antigens / physiology* CHO Cells Cholesterol / metabolism* Cricetinae Endocytosis / drug effects Endocytosis / physiology* Glycation End Products, Advanced / pharmacokinetics* Iodine Radioisotopes / pharmacokinetics Kinetics Lipoproteins, HDL / metabolism Lipoproteins, LDL / pharmacology Liver / cytology Liver / metabolism* Male Membrane Proteins* Rats Rats, Wistar Receptors, Immunologic* Receptors, Lipoprotein / physiology Receptors, Scavenger Recombinant Proteins / metabolism Scavenger Receptors, Class A Scavenger Receptors, Class B Serum Albumin, Bovine / pharmacokinetics* |
| IF | 4.238 |
| Times Cited | 122 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CHO-K1(RCB0285) Hep G2(RCB0459) |