RRC ID 41358
著者 Nozawa K, Maehara K, Isobe K.
タイトル Mechanism for the reduction of telomerase expression during muscle cell differentiation.
ジャーナル J Biol Chem
Abstract Telomerase, the reverse transcriptase that maintains telomere DNA, is usually undetectable in adult human tissues, but is positive in embryonic tissues and in cancers. However, in rodents, several organs of normal adult animals express substantial amounts of telomerase activity. To elucidate relevant control mechanisms operating on the tissue-specific expression of telomerase in rodents, we examined the transcriptional regulation of telomerase reverse transcriptase (mTERT) gene in muscle cell differentiation. Reverse transcriptase-polymerase chain reaction analysis showed that the reduction of telomerase activity was caused by the decrease of mTERT mRNA level during myogenesis. Transfections of mTERT promoter showed that the proximal 225-base pair region is the core promoter responsible for basal transcriptional activity and also participates in the reduced transcription after muscle differentiation. Electrophoretic mobility shift assays showed that this region contained the GC-boxes, which bind to Sp1 family proteins, and the E-box, which binds to c-Myc. Furthermore, DNA binding activities of Sp1, Sp3, and c-Myc were down-regulated during myogenesis. These data suggest that Sp1, Sp3, and c-Myc have critical roles of TERT transactivation in mouse, and the lack of these transcription factors cause down-regulation of mTERT gene expression in muscle cells differentiation.
巻・号 276(25)
ページ 22016-23
公開日 2001-6-22
DOI 10.1074/jbc.M011181200
PII S0021-9258(20)78467-X
PMID 11279234
MeSH 3T3 Cells Animals Base Sequence Cell Differentiation* DNA DNA-Binding Proteins Gene Expression Regulation Mice Molecular Sequence Data Muscle Development Muscles / cytology Muscles / metabolism* Promoter Regions, Genetic RNA* RNA, Messenger / genetics RNA, Messenger / metabolism Telomerase / antagonists & inhibitors* Telomerase / genetics Telomerase / metabolism Transcription, Genetic Transfection
IF 4.238
引用数 34
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 C2C12(RCB0987) NIH3T3 H10T1/2(RCB0247)