論文 - 詳細
| RRC ID | 41384 |
|---|---|
| 著者 | Onoue S, Waki Y, Nagano Y, Satoh S, Kashimoto K. |
| タイトル | The neuromodulatory effects of VIP/PACAP on PC-12 cells are associated with their N-terminal structures. |
| ジャーナル | Peptides |
| Abstract |
ONOUE, S., WAKI, Y., NAGANO, Y., SATOH, S., KASHIMOTO, K. Neuromodulatory Effects of VIP/PACAP on PC-12 Cells Are Associated with Their N-terminal Structures. PEPTIDES xx(xx) 000-000, 200x.- The current study explored whether the differences in biological activities in PC-12 cells between vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are attributable to the sequence difference in their N-terminal portions and are correlated with the solution structures of the peptides. In the neurite outgrowth assay, N-terminal modification of VIP to PACAP-like sequences altered its effect, the activity was confirmed even at a low concentration (10(-10) M). On the contrary, N-terminal modification of PACAP 27 to VIP-like sequences reduced its activity. These relationships were also confirmed for the inhibitory effects of the peptide analogues on PC-12 cells growth at 10(-7) M. The present results combined with our previously reported data, including binding assay, support that the N-termini of VIP/PACAP plays an important role in their activities. |
| 巻・号 | 22(6) |
| ページ | 867-72 |
| 公開日 | 2001-6-1 |
| DOI | 10.1016/s0196-9781(01)00411-9 |
| PII | S0196-9781(01)00411-9 |
| PMID | 11390015 |
| MeSH | Amino Acid Sequence Animals Cell Division Dimethyl Sulfoxide / pharmacology Dose-Response Relationship, Drug Fluorescent Dyes / pharmacology* Magnetic Resonance Spectroscopy Models, Molecular Molecular Sequence Data Neurons / cytology Neuropeptides / pharmacology* PC12 Cells Peptide Biosynthesis Pituitary Adenylate Cyclase-Activating Polypeptide Protein Structure, Tertiary Rats Sequence Homology, Amino Acid Vasoactive Intestinal Peptide / chemistry |
| IF | 2.843 |
| 引用数 | 29 |
| WOS 分野 | PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |