論文 - 詳細
| RRC ID | 41385 |
|---|---|
| 著者 | Fukushima K, Hara-Kuge S, Ideo H, Yamashita K. |
| タイトル | Carbohydrate recognition site of interleukin-2 in relation to cell proliferation. |
| ジャーナル | J Biol Chem |
| Abstract |
Interleukin-2 (IL-2) is a cytokine with important roles in the immune system. IL-2 initially binds a high mannose-type glycan and a specific peptide sequence of the IL-2 receptor alpha-subunit and sequentially forms a high affinity complex of IL-2.IL-2 receptor alpha-, beta-, and gamma-subunits. This formation induces cellular signaling and cell proliferation (Fukushima, K., and Yamashita, K. (2001) J. Biol. Chem. 276, 7351-7356). To determine the carbohydrate-binding site of IL-2, we prepared wild-type and point-mutated (35)S-IL-2 by an in vitro transcription and translation method. We found that wild-type (35)S-IL-2 tends to form a dimer spontaneously, and the dimeric form has both carbohydrate recognition activity and cell proliferation activity. Moreover, substitution of Asn-26 in IL-2 with Gln or Asp conserved the dimeric form and affected the carbohydrate recognition activities in correspondence with the cell proliferation activities, suggesting that Asn-26 in IL-2 is involved in the carbohydrate recognition site. These results suggest that the carbohydrate recognition of IL-2 dimer triggers formation of high affinity complex (IL-2.IL-2Ralpha, -beta, -gamma)(2), and the hetero-octamer stimulates IL-2-dependent T-cell proliferation by intensifying cellular signaling. |
| 巻・号 | 276(33) |
| ページ | 31202-8 |
| 公開日 | 2001-8-17 |
| DOI | 10.1074/jbc.M102789200 |
| PII | S0021-9258(20)80274-9 |
| PMID | 11390392 |
| MeSH | Animals Binding Sites Cell Division / drug effects Cell Line Dimerization Interleukin-2 / chemistry* Interleukin-2 / metabolism Interleukin-2 / pharmacology Mice Point Mutation Polysaccharides / metabolism* Receptors, Interleukin-2 / metabolism Recombinant Proteins / chemistry Recombinant Proteins / metabolism |
| IF | 4.238 |
| 引用数 | 13 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CTLL-2(RCB0637) |