Reference - Detail
| RRC ID | 41400 |
|---|---|
| Author | Takahashi Y, Tanaka Y, Yamashita A, Koyanagi Y, Nakamura M, Yamamoto N. |
| Title | OX40 stimulation by gp34/OX40 ligand enhances productive human immunodeficiency virus type 1 infection. |
| Journal | J Virol |
| Abstract |
OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T cells. To investigate the role of costimulation of OX40 in human immunodeficiency virus type 1 (HIV-1) infection by its natural ligand, gp34, the OX40-transfected ACH-2 cell line, ACH-2/OX40, chronically infected with HIV-1, was cocultured with paraformaldehyde (PFA)-fixed gp34-transfected mouse cell line, SV-T2/gp34. The results showed that HIV-1 production was strongly induced. This was followed by apparent apoptosis, and both processes were specifically inhibited by the gp34-specific neutralizing monoclonal antibody 5A8. Endogenous TNF alpha (TNF-alpha) and TNF-beta production were not involved in the enhanced HIV-1 production. Furthermore, enhanced HIV-1 transcription in gp34-stimulated ACH-2/OX40 cells was dependent on the kappa B site of the HIV-1 long terminal repeat, and the OX40-gp34 interaction activated NF-kappa B consisting of p50 and p65 subunits. When primary activated CD4(+) T cells acutely infected with HIV-1(NL4-3) (CXCR4-using T-cell-line-tropic) were cocultured with PFA-fixed gp34(+) human T-cell leukemia virus type 1-bearing MT-2 cells or SV-T2/gp34 cells, HIV-1 production was also markedly enhanced. The enhancement was again significantly inhibited by 5A8. The present study first shows that OX40-gp34 interaction stimulates HIV-1 expression and suggests that OX40 triggering by gp34 may play an important role in enhancing HIV-1 production in both acutely and latently infected CD4(+) T cells in vivo. |
| Volume | 75(15) |
| Pages | 6748-57 |
| Published | 2001-8-1 |
| DOI | 10.1128/JVI.75.15.6748-6757.2001 |
| PMID | 11435553 |
| PMC | PMC114401 |
| MeSH | Animals Apoptosis Binding Sites CD4-Positive T-Lymphocytes / cytology CD4-Positive T-Lymphocytes / metabolism CD4-Positive T-Lymphocytes / virology* Cell Line Cells, Cultured Gene Expression Regulation, Viral HIV Long Terminal Repeat HIV-1 / metabolism HIV-1 / physiology* Human T-lymphotropic virus 1 Humans Lymphotoxin-alpha / metabolism Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Mice Mice, Inbred BALB C NF-kappa B / metabolism OX40 Ligand Receptors, OX40 Receptors, Tumor Necrosis Factor* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Transcription, Genetic Tumor Necrosis Factor Receptor Superfamily, Member 7 / genetics Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism* Tumor Necrosis Factor-alpha / metabolism Tumor Necrosis Factors |
| IF | 4.501 |
| Times Cited | 26 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | SP2/0-Ag14(RCB0209) |