論文 - 詳細
| RRC ID | 41405 |
|---|---|
| 著者 | Arima H, Kihara F, Hirayama F, Uekama K. |
| タイトル | Enhancement of gene expression by polyamidoamine dendrimer conjugates with alpha-, beta-, and gamma-cyclodextrins. |
| ジャーナル | Bioconjug Chem |
| Abstract |
To improve the transfection efficiency of nonviral vector, we synthesized the starburst polyamidoamine dendrimer conjugates with alpha-, beta-, and gamma-cyclodextrins (CDE conjugates), expecting the synergistic effect of dendrimer and cyclodextrins (CyDs). The (1)H NMR spectroscopic data indicated that alpha-, beta-, and gamma-CyDs are covalently bound to dendrimer in a molar ratio of 1:1. The agarose gel electrophoretic studies revealed that CDE conjugates formed the complexes with plasmid DNA (pDNA) and protected the degradation of pDNA by DNase I in the same manner as dendrimer. CDE conjugates showed a potent luciferase gene expression, especially in the dendrimer conjugate with alpha-CyD (alpha-CDE conjugate) which provided the greatest transfection activity (approximately 100 times higher than those of dendrimer alone and of the physical mixture of dendrimer and alpha-CyD) in NIH3T3 and RAW264.7 cells. In addition, the gene transfer activity of alpha-CDE conjugate was superior to that of Lipofectin. The enhancing gene transfer effect of alpha-CDE conjugate may be attributable to not only increasing the cellular association, but also changing the intracellular trafficking of pDNA. These findings suggest that alpha-CDE conjugate could be a new preferable nonviral vector of pDNA. |
| 巻・号 | 12(4) |
| ページ | 476-84 |
| 公開日 | 2001-1-1 |
| DOI | 10.1021/bc000111n |
| PII | bc000111n |
| PMID | 11459450 |
| MeSH | 3T3 Cells / metabolism Animals Binding Sites / physiology Cyclodextrins / chemistry Cyclodextrins / pharmacology* DNA / chemistry DNA / metabolism* Deoxyribonuclease I / metabolism* Drug Synergism Gene Expression / drug effects* Gene Transfer Techniques Genetic Vectors / genetics Mice Microscopy, Confocal Phosphatidylethanolamines / pharmacology Plasmids / chemistry Polymers / pharmacology* Transfection |
| IF | 4.031 |
| 引用数 | 198 |
| WOS 分野 | CHEMISTRY, ORGANIC BIOCHEMICAL RESEARCH METHODS CHEMISTRY, MULTIDISCIPLINARY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |