論文 - 詳細
| RRC ID | 41406 |
|---|---|
| 著者 | Kiriyama Y, Tsuchiya H, Murakami T, Satoh K, Tokumitsu Y. |
| タイトル | Calcitonin induces IL-6 production via both PKA and PKC pathways in the pituitary folliculo-stellate cell line. |
| ジャーナル | Endocrinology |
| Abstract |
It has been demonstrated that calcitonin-binding sites are present in a variety of tissue types, including in the pituitary gland. Interleukin-6 (IL-6) is also produced in the pituitary and it regulates the secretion of various hormones. In this study, we examined the expression of the calcitonin receptor and the mechanism of IL-6 production induced by calcitonin in the pituitary folliculo-stellate cell line (TtT/GF). The mRNA of calcitonin receptor subtype C1a, but not that of C1b, was detected by RT-PCR in TtT/GF cells and in the normal mouse pituitary. Calcitonin increased cAMP accumulation and IL-6 production in a concentration-dependent manner in TtT/GF cells. As calcitonin activates the PKA and PKC pathways, we investigated the contributions of PKA and PKC to IL-6 production. IL-6 production was only slightly increased by either 8-bromo-cAMP (1 mM) or phorbol 12-myristate 13-acetate (100 nM) alone. However, IL-6 was synergistically induced in the presence of both 8-bromo-cAMP (1 mM) and phorbol 12myristate 13-acetate (100 nM). Furthermore, calcitonin-induced IL-6 production was completely suppressed by H-89 (PKA inhibitor) or GF109203X (PKC inhibitor), indicating that the activation of both PKA and PKC is necessary for calcitonin-induced IL-6 production. On the other hand, pertussis toxin (G(i)/G(o) signaling inhibitor) treatment achieved an approximately 9-fold increase in calcitonin-induced IL-6 production. These results show that calcitonin-stimulated IL-6 production is mediated via both PKA- and PKC-signaling pathways, whereas calcitonin also suppresses IL-6 production by activating G(i)/G(o) proteins in folliculo-stellate cells. |
| 巻・号 | 142(8) |
| ページ | 3563-9 |
| 公開日 | 2001-8-1 |
| DOI | 10.1210/endo.142.8.8328 |
| PMID | 11459804 |
| MeSH | Animals Calcitonin / pharmacology* Cell Line Cyclic AMP / metabolism Cyclic AMP-Dependent Protein Kinases / metabolism* Enzyme Inhibitors / pharmacology GTP-Binding Proteins / physiology Interleukin-6 / antagonists & inhibitors Interleukin-6 / biosynthesis* Male Mice Mice, Inbred BALB C Pituitary Gland / cytology Pituitary Gland / drug effects* Pituitary Gland / metabolism* Protein Kinase C / metabolism* RNA, Messenger / metabolism Receptors, Calcitonin / genetics Signal Transduction / drug effects |
| IF | 3.934 |
| 引用数 | 17 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | TtT/GF(RCB1279) |