論文 - 詳細
| RRC ID | 41409 |
|---|---|
| 著者 | Satoh JI, Kuroda Y. |
| タイトル | Alpha-synuclein expression is up-regulated in NTera2 cells during neuronal differentiation but unaffected by exposure to cytokines and neurotrophic factors. |
| ジャーナル | Parkinsonism Relat Disord |
| Abstract |
Increasing evidence has indicated that proinflammatory cytokines such as TNF-alpha and IL-1beta, produced by activated microglia and astrocytes, play a key role in progressive degeneration of the nigrostriatal dopaminergic neurons in Parkinson's disease (PD). Since alpha-synuclein is a major component of Lewy bodies in PD brains, we studied the constitutive and cytokine/neurotrophic factor-regulated expression of alpha-synuclein in cultured human neurons by Northern blot and Western blot analyses. The constitutive expression of alpha-synuclein mRNA was identified in a variety of human neural and non-neural cell lines. The levels of alpha-synuclein expression were elevated markedly in NTera2 teratocarcinoma cells following retinoic acid-induced neuronal differentiation, accompanied with an increased expression of synphilin-1, while they were unaltered in NTera2-derived differentiated neurons by exposure to TNF-alpha, IL-1beta, BDNF or GDNF. These results indicate that alpha-synuclein expression in human neurons is up-regulated during differentiation, but is unaffected by a panel of cytokines and neurotrophic factors which are supposed to be involved in the nigral neuronal death and survival. |
| 巻・号 | 8(1) |
| ページ | 7-17 |
| 公開日 | 2001-9-1 |
| DOI | 10.1016/s1353-8020(00)00075-4 |
| PII | S1353-8020(00)00075-4 |
| PMID | 11472875 |
| MeSH | Blotting, Northern Blotting, Southern Blotting, Western Brain-Derived Neurotrophic Factor / pharmacology Cell Death / drug effects Cell Differentiation / genetics Cell Differentiation / physiology Cell Line Cell Survival / drug effects Cytokines / pharmacology* Glial Cell Line-Derived Neurotrophic Factor Humans Interleukin-1 / pharmacology Nerve Growth Factors / pharmacology* Nerve Tissue Proteins / biosynthesis* Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / pharmacology Neurons / metabolism* Reverse Transcriptase Polymerase Chain Reaction Synucleins Teratocarcinoma / metabolism* Tumor Cells, Cultured Tumor Necrosis Factor-alpha / pharmacology Up-Regulation / drug effects Up-Regulation / genetics Up-Regulation / physiology* alpha-Synuclein |
| IF | 3.926 |
| 引用数 | 19 |
| WOS 分野 | CLINICAL NEUROLOGY |
| リソース情報 | |
| ヒト・動物細胞 | KG-1-C(RCB0270) HeLa(RCB0007) A549(RCB0098) MOLT-4(RCB0206) Hep G2(RCB0459) |