RRC ID 41419
著者 Lee DH, Park JC, Suh H.
タイトル Effect of centrifugal force on cellular activity of osteoblastic MC3T3-E1 cells in vitro.
ジャーナル Yonsei Med J
Abstract The effects of centrifugal force on growth and differentiation of osteoblastic cells cultured in alpha-MEM containing 1% Fetal bovine serum were investigated by assays of DNA synthesis, alkaline phosphatase activity and osteocalcin-production in osteoblastic MC3T3-E1 cells. Centrifugation of the cells in low concentrations (1%) of fetal bovine serum caused a 1.9-fold increase of [3H]thymidine incorporation on day 3 from the start of centrifugation, and gradually decreased with culture up to day 9. Alkaline phosphatase activity was not affected by centrifugal force until day 5, and increased rapidly after day 7. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. These results suggest that centrifugal force stimulates the proliferation of osteoblastic cells through an autocrine secretion of some diffusable growth- promoting activity. Additional centrifugation of the cells also slightly stimulated alkaline phosphatase activity, although this did not directly influence the cell's osteocalcin-production activity.
巻・号 42(4)
ページ 405-10
公開日 2001-8-1
DOI 10.3349/ymj.2001.42.4.405
PII 200108405
PMID 11519082
MeSH Alkaline Phosphatase / metabolism Animals Cell Division Cells, Cultured Centrifugation DNA / biosynthesis Mice Osteoblasts / physiology* Stress, Mechanical
IF 1.914
引用数 9
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)