Reference - Detail
| RRC ID | 41430 |
|---|---|
| Author | Nagase M, Alam MM, Tsushima A, Yoshizawa T, Sakato N. |
| Title | Apoptosis induction by T-2 toxin: activation of caspase-9, caspase-3, and DFF-40/CAD through cytosolic release of cytochrome c in HL-60 cells. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
The molecules participating in apoptosis induced by T-2 toxin in human leukemia HL-60 cells were investigated. The rank order of the potency of trichothecene mycotoxins to induce internucleosomal DNA fragmentation was found to be T-2, satratoxin G, roridin A > diacetoxyscirpenol > baccharin B-5 > nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenon-X, baccharin B-4=vehicle control. Western blot analysis of caspase-3 in T-2-treated cells clearly indicated the appearance of its catalytically active fragment of 17-kDa. Increased caspase-3 activity was also detected by using a fluorogenic substrate, DEVD-AMC. Next, cells exposed to T-2 led to cleavage of PARP from its native 116-kDa form to the 85-kDa product. Moreover, DFF-45/ICAD were cleaved to give a 12.5-kDa fragment via T-2 treatment. T-2 caused the release of cytochrome c from mitochondria into the cytosol. Increased enzymic activity of caspase-9 on LEHD-AMC was shown. These data indicate that T-2-induced apoptosis involves activation of caspase-3 and DFF-40/CAD through cytosolic accumulation of cytochrome c along with caspase-9 activation. |
| Volume | 65(8) |
| Pages | 1741-7 |
| Published | 2001-8-1 |
| DOI | 10.1271/bbb.65.1741 |
| PMID | 11577712 |
| MeSH | Apoptosis / drug effects* Blotting, Western Caspase 3 Caspase 9 Caspases / metabolism* Cytochrome c Group / metabolism* Cytosol / drug effects Cytosol / enzymology* DNA Fragmentation Deoxyribonucleases / metabolism* Electrophoresis, Polyacrylamide Gel Enzyme Activation / drug effects HL-60 Cells Humans Indicators and Reagents Mitochondria / drug effects Mitochondria / enzymology Nucleosomes / chemistry Nucleosomes / drug effects Poly-ADP-Ribose Binding Proteins T-2 Toxin / toxicity* |
| IF | 1.516 |
| Times Cited | 48 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Policy sources |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |