Reference - Detail
| RRC ID | 41444 |
|---|---|
| Author | Dekigai H, Nakamura H, Bai J, Tanito M, Masutani H, Hirota K, Matsui H, Murakami M, Yodoi J. |
| Title | Geranylgeranylacetone promotes induction and secretion of thioredoxin in gastric mucosal cells and peripheral blood lymphocytes. |
| Journal | Free Radic Res |
| Abstract |
Thioredoxin (TRX) is a redox-active protein which is induced by oxidative stresses and shows a variety of biological activities including cytoprotection against oxidative stress. We recently reported that geranylgeranylacetone (GGA), an anti-ulcer drug, induces TRX in rat hepatocytes. In this study, we demonstrate that GGA promotes induction and secretion of TRX in rat gastric mucosal cells and human peripheral blood lymphocytes (PBLs). Western blotting and a sensitive sandwich ELISA showed that TRX was induced by GGA in the cell lysates and culture supernatants of rat gastric mucosal RGM-1 cells and human PBLs. LDH releasing assay showed that GGA protected rat gastric mucosal RGM-1 cells from ethanol-induced cytotoxicity. Moreover, exogenous recombinant wild type TRX decreased 51Cr release from primary cultured rat gastric mucosal cells incubated with ethanol or hydrogen peroxide in a dose-dependent manner, whereas recombinant mutant TRX (C32S/C35S), in which the two cysteines were replaced with serines in its active site, did not. These results indicate that GGA promotes the induction and secretion of TRX in a variety of types of cells and suggest that induced or secreted TRX may play an important role in the cytoprotective action of GGA on gastric mucosal cells. |
| Volume | 35(1) |
| Pages | 23-30 |
| Published | 2001-7-1 |
| DOI | 10.1080/10715760100300561 |
| PMID | 11697114 |
| MeSH | Animals Anti-Ulcer Agents / pharmacology* Blotting, Western Cell Line Cytoprotection Diterpenes / pharmacology* Enzyme-Linked Immunosorbent Assay Gastric Mucosa / drug effects* Gastric Mucosa / metabolism Humans L-Lactate Dehydrogenase / metabolism Lymphocytes / drug effects* Lymphocytes / metabolism Rats Thioredoxins / metabolism* |
| IF | 2.839 |
| Times Cited | 30 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RGM1(RCB0876) |