| RRC ID |
41449
|
| Author |
Soeda S, Ochiai T, Paopong L, Tanaka H, Shoyama Y, Shimeno H.
|
| Title |
Crocin suppresses tumor necrosis factor-alpha-induced cell death of neuronally differentiated PC-12 cells.
|
| Journal |
Life Sci
|
| Abstract |
Crocus sativus L. is used in Chinese traditional medicine to treat some disorders of the central nervous system. Crocin is an ethanol-extractable component of Crocus sativus L.; it is reported to prevent ethanol-induced impairment of learning and memory in mice. In this study, we demonstrate that crocin suppresses the effect of tumor necrosis factor (TNF)-alpha on neuronally differentiated PC-12 cells. PC-12 cells dead from exposure to TNF-alpha show apoptotic morphological changes and DNA fragmentation. These hallmark features of cell death did not appear in cells treated in the co-presence of 10 microM crocin. Moreover, crocin suppressed the TNF-alpha-induced expression of Bcl-Xs and LICE mRNAs and simultaneously restored the cytokine-induced reduction of Bcl-X(L) mRNA expression. The modulating effects of crocin on the expression of Bcl-2 family proteins led to a marked reduction of a TNF-alpha-induced release of cytochrome c from the mitochondria. Crocin also blocked the cytochrome c-induced activation of caspase-3. To learn how crocin exhibits these anti-apoptotic actions in PC-12 cells, we tested the effect of crocin on PC-12 cell death induced by daunorubicin. We found that crocin inhibited the effect of daunorubicin as well. Our findings suggest that crocin inhibits neuronal cell death induced by both internal and external apoptotic stimuli.
|
| Volume |
69(24)
|
| Pages |
2887-98
|
| Published |
2001-11-2
|
| DOI |
10.1016/s0024-3205(01)01357-1
|
| PII |
S0024-3205(01)01357-1
|
| PMID |
11720092
|
| MeSH |
Animals
Apoptosis / drug effects*
Carotenoids / pharmacology*
Caspase 1 / biosynthesis
Caspase 1 / genetics
Caspase 3
Caspases / metabolism
Cytochrome c Group / immunology
Cytochrome c Group / metabolism
DNA Fragmentation / drug effects
Daunorubicin / pharmacology
Dose-Response Relationship, Drug
Drug Antagonism
Drugs, Chinese Herbal / pharmacology*
Enzyme-Linked Immunosorbent Assay
Mitochondria / drug effects
Mitochondria / enzymology
Neurons / drug effects*
Neurons / pathology
PC12 Cells / drug effects
PC12 Cells / pathology
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Proto-Oncogene Proteins c-bcl-2 / genetics
RNA, Messenger / metabolism
Rats
Tumor Necrosis Factor-alpha / pharmacology*
bcl-X Protein
|
| IF |
3.647
|
| Times Cited |
113
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
MEDICINE, RESEARCH & EXPERIMENTAL
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
