| RRC ID |
41457
|
| 著者 |
Chung H, Kang YS, Hwang CS, Moon IK, Yim CH, Choi KH, Han KO, Jang HC, Yoon HK, Han IK.
|
| タイトル |
Deflazacort increases osteoclast formation in mouse bone marrow culture and the ratio of RANKL/OPG mRNA expression in marrow stromal cells.
|
| ジャーナル |
J Korean Med Sci
|
| Abstract |
Information on precise effects of deflazacort on bone cell function, especially osteoclasts, is quite limited. Therefore, the present study was undertaken to test effects of deflazacort on osteoclast-like cell formation in mouse bone marrow cultures and on the regulation of osteoprotegerin (OPG) and its ligand (RANKL) mRNA expressions by RT-PCR in the ST2 marrow stromal cells. TRAP-positive mononuclear cells increased after the treatment of deflazacort at 10(-9) to 10(-7) M alone for 6 days in a dose-dependent manner. Number of TRAP-positive multi-nucleated cells (MNCs) increased significantly with combined treatment of deflazacort at 10(-7) M and 1,25-(OH)2D3 at 10(-9) M compared to that of cultures treated with 1,25-(OH)2D3 alone (p<0.05). Exposure to deflazacort at 10(-7) M in the presence of 1,25-(OH)2D3 at 10(-9) M in the last 3-day culture had greater stimulatory effect on osteoclast-like cell formation than that of the first 3-day culture did. Deflazacort at 10(-10) -10(-6) M downregulated OPG and upregulated RANKL in mRNA levels in a dose-dependent manner. These observations suggest that deflazacort stimulate osteoclast precursor in the absence of 1,25-(OH)2D3 and enhance differentiation of osteoclasts in the presence of 1,25-(OH)2D3. These effects are, in part, thought to be mediated by the regulation of the expression of OPG and RANKL mRNA in marrow stromal cells.
|
| 巻・号 |
16(6)
|
| ページ |
769-73
|
| 公開日 |
2001-12-1
|
| DOI |
10.3346/jkms.2001.16.6.769
|
| PII |
200112769
|
| PMID |
11748360
|
| PMC |
PMC3054787
|
| MeSH |
Animals
Bone Marrow Cells / cytology*
Calcitriol / pharmacology
Calcium Channel Agonists / pharmacology
Carrier Proteins / genetics*
Cell Differentiation / drug effects
Cells, Cultured
Dexamethasone / pharmacology
Gene Expression / drug effects
Glucocorticoids / pharmacology
Glycoproteins / genetics*
Immunosuppressive Agents / pharmacology*
Male
Membrane Glycoproteins / genetics*
Mice
Mice, Inbred ICR
Osteoclasts / cytology*
Osteoprotegerin
Pregnenediones / pharmacology*
RANK Ligand
RNA, Messenger / analysis
Receptor Activator of Nuclear Factor-kappa B
Receptors, Cytoplasmic and Nuclear / genetics*
Receptors, Tumor Necrosis Factor
Stromal Cells / cytology
|
| IF |
1.705
|
| 引用数 |
10
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
