RRC ID 4146
Author Higashijima S, Mandel G, Fetcho JR.
Title Distribution of prospective glutamatergic, glycinergic, and GABAergic neurons in embryonic and larval zebrafish.
Journal J Comp Neurol
Abstract Zebrafish are an excellent model for studies of the functional organization of neuronal circuits, but little is known regarding the transmitter phenotypes of the neurons in their nervous system. We examined the distribution in spinal cord and hindbrain of neurons expressing markers of transmitter phenotype, including the vesicular glutamate transporter (VGLUT) genes for glutamatergic neurons, the neuronal glycine transporter (GLYT2) for glycinergic neurons, and glutamic acid decarboxylase (GAD65/67) for GABAergic neurons. All three markers were expressed in a large domain in the dorsal two-thirds of spinal cord, with additional, more ventral expression domains for VGLUT2 and GAD/GABA. In the large dorsal domain, dual in situ staining showed that GLYT2-positive cells were intermingled with VGLUT2 cells, with no dual-stained neurons. Many of the neurons in the dorsal expression domain that were positive for GABA markers at embryonic stages were also positive for GLYT2, suggesting that the cells might use both GABA and glycine, at least early in their development. The intermingling of neurons expressing inhibitory and excitatory markers in spinal cord contrasted markedly with the organization in hindbrain, where neurons expressing a particular marker were clustered together to form stripes that were visible running from rostral to caudal in horizontal sections and from dorsomedial to ventrolateral in cross sections. Dual labeling showed that the stripes of neurons labeled with one transmitter marker alternated with stripes of cells labeled for the other transmitter phenotypes. The differences in the distribution of excitatory and inhibitory neurons in spinal cord versus hindbrain may be tied to differences in their patterns of development and functional organization.
Volume 480(1)
Pages 1-18
Published 2004-11-29
DOI 10.1002/cne.20278
PMID 15515020
MeSH Amino Acid Transport Systems, Neutral / metabolism Animals Biomarkers Central Nervous System / embryology* Central Nervous System / growth & development Central Nervous System / metabolism Glutamate Decarboxylase / metabolism Glutamic Acid / metabolism* Glycine / metabolism* Glycine Plasma Membrane Transport Proteins Immunohistochemistry Isoenzymes / metabolism Larva / growth & development Larva / metabolism Membrane Transport Proteins / metabolism Molecular Sequence Data Neural Inhibition / physiology Neurons / cytology Neurons / metabolism* Rhombencephalon / embryology Rhombencephalon / growth & development Rhombencephalon / metabolism Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Spinal Cord / embryology Spinal Cord / growth & development Spinal Cord / metabolism Synaptic Transmission / physiology Vesicular Glutamate Transport Protein 1 Zebrafish / embryology* Zebrafish / growth & development Zebrafish / metabolism gamma-Aminobutyric Acid / metabolism*
IF 3.239
Times Cited 144
Zebrafish ?