論文 - 詳細
| RRC ID | 41466 |
|---|---|
| 著者 | Mu MM, Chakravortty D, Sugiyama T, Koide N, Takahashi K, Mori I, Yoshida T, Yokochi T. |
| タイトル | The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. |
| ジャーナル | J Endotoxin Res |
| Abstract |
The effect of quercetin on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was studied. Quercetin pretreatment significantly inhibited NO production in an LPS-stimulated RAW 264.7 murine macrophage cell line. Post-treatment with quercetin partially inhibited NO production. The inhibitory action of quercetin was due to neither the cytotoxic action nor altered LPS binding. The expression of inducible-type NO synthase (iNOS) was markedly down-regulated by quercetin. Quercetin suppressed the release of free nuclear factor (NF)-kappaB by preventing degradation of IkappaB-alpha and IkappaB-beta. Moreover, quercetin blocked the phosphorylation of extracellular signal regulated kinase 1/2 (Erk 1/2), p38, and c-Jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK) and, further, the activity of tyrosine kinases in LPS-stimulated RAW cells. Quercetin also inhibited interferon (IFN)-gamma-induced NO production. Taken together, these results indicate that the inhibitory action of quercetin on NO production in LPS- and/or IFN-gamma-stimulated macrophages might be due to abrogation of iNOS protein induction by impairment of a series of intracellular signal pathways. |
| 巻・号 | 7(6) |
| ページ | 431-8 |
| 公開日 | 2001-1-1 |
| DOI | 10.1179/096805101101533034 |
| PMID | 11753212 |
| MeSH | Animals Antioxidants / pharmacology* Cell Line Down-Regulation Enzyme Induction Enzyme Inhibitors / pharmacology I-kappa B Proteins / antagonists & inhibitors Immunoblotting In Vitro Techniques Interferon-gamma / antagonists & inhibitors JNK Mitogen-Activated Protein Kinases Lipopolysaccharides / antagonists & inhibitors* Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / enzymology Macrophages / metabolism* Mice Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism Nitric Oxide / biosynthesis* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Phosphorylation Quercetin / pharmacology* p38 Mitogen-Activated Protein Kinases |
| 引用数 | 53 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |