RRC ID 41483
Author Masuoka M, Yoshimuta T, Hamada M, Okamoto M, Fumimori T, Honda J, Oizumi K, Itoh K.
Title Identification of the HLA-A24 peptide epitope within cytomegalovirus protein pp65 recognized by CMV-specific cytotoxic T lymphocytes.
Journal Viral Immunol.
Abstract Among cytomegalovirus (CMV) tegument proteins, phosphoprotein 65 (pp65) has been identified as the important target antigen of the cytotoxic T lymphocyte (CTL) response against the virus. We synthesized seven CMV-pp65-derived peptides carrying an HLA-A24-binding motif, and investigated the ability of these peptides to induce CMV-specific CTL. We identified one nonamer peptide (pp65113-121; VYALPLKML) able to bind HLA-A24 and induce CTL responses in vitro in peripheral blood mononuclear cells (PBMC) from CMV-seropositive individuals. The peptide-specific CTLs generated were capable of recognizing pp65 expressed on CMV-infected fibroblasts as well as pp65113-121 peptide bound to the surface of C1R-A*2402 cells in an HLA-A24-restricted manner. The pp65113-121 peptide thus might be considered a synthetic peptide vaccine in HLA-A24-positive individuals.
Volume 14(4)
Pages 369-77
Published 2001
DOI 10.1089/08828240152716619
PMID 11792066
MeSH Antigens, Viral / immunology Cells, Cultured Cytomegalovirus / immunology* Epitopes, T-Lymphocyte / immunology* HLA-A Antigens / analysis* HLA-A Antigens / immunology HLA-A24 Antigen Humans Peptides / chemical synthesis Peptides / immunology Phosphoproteins / chemical synthesis Phosphoproteins / immunology* T-Lymphocytes, Cytotoxic / immunology* Viral Matrix Proteins / chemical synthesis Viral Matrix Proteins / immunology*
IF 1.417
Times Cited 26
Human and Animal Cells