RRC ID 4149
Author Cooper KL, Leisenring WM, Moens CB.
Title Autonomous and nonautonomous functions for Hox/Pbx in branchiomotor neuron development.
Journal Dev. Biol.
Abstract The vertebrate branchiomotor neurons are organized in a pattern that corresponds with the segments, or rhombomeres, of the developing hindbrain and have identities and behaviors associated with their position along the anterior/posterior axis. These neurons undergo characteristic migrations in the hindbrain and project from stereotyped exit points. We show that lazarus/pbx4, which encodes an essential Hox DNA-binding partner in zebrafish, is required for facial (VIIth cranial nerve) motor neuron migration and for axon pathfinding of trigeminal (Vth cranial nerve) motor axons. We show that lzr/pbx4 is required for Hox paralog group 1 and 2 function, suggesting that Pbx interacts with these proteins. Consistent with this, lzr/pbx4 interacts genetically with hoxb1a to control facial motor neuron migration. Using genetic mosaic analysis, we show that lzr/pbx4 and hoxb1a are primarily required cell-autonomously within the facial motor neurons; however, analysis of a subtle non-cell-autonomous effect indicates that facial motor neuron migration is promoted by interactions amongst the migrating neurons. At the same time, lzr/pbx4 is required non-cell-autonomously to control the pathfinding of trigeminal motor axons. Thus, Pbx/Hox can function both cell-autonomously and non-cell-autonomously to direct different aspects of hindbrain motor neuron behavior.
Volume 253(2)
Pages 200-13
Published 2003-1-15
PII S0012-1606(02)00018-0
PMID 12645925
MeSH Animals Animals, Genetically Modified Cell Movement / genetics DNA-Binding Proteins* Facial Nerve / embryology Gene Targeting Genes, Homeobox* Glycosyltransferases / genetics Green Fluorescent Proteins Homeodomain Proteins / genetics Luminescent Proteins / genetics Mosaicism Motor Neurons / cytology* Neural Pathways / embryology Rhombencephalon / embryology Trigeminal Nerve / embryology Zebrafish / genetics* Zebrafish / growth & development* Zebrafish Proteins / genetics
IF 3.262
Times Cited 45
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Zebrafish rw0 (Tg(CM-isl1:GFP))