Reference - Detail
RRC ID | 41534 |
---|---|
Author | Ko JH, Jin HK, Asano A, Takada A, Ninomiya A, Kida H, Hokiyama H, Ohara M, Tsuzuki M, Nishibori M, Mizutani M, Watanabe T. |
Title | Polymorphisms and the differential antiviral activity of the chicken Mx gene. |
Journal | Genome Res |
Abstract |
The nucleotide sequence of chicken Mx cDNA was reported earlier using the White Leghorn breed in Germany, but it showed no enhanced resistance to viruses. In this study, the nucleotide sequences of chicken Mx cDNA were determined in many breeds. A total of 25 nucleotide substitutions, of which 14 were deduced to cause amino acid exchanges, were detected, suggesting that the chicken Mx gene is very polymorphic. Transfected cell clones expressing chicken Mx mRNA were established after the Mx cDNA was constructed with an expression vector and introduced into mouse 3T3 cells, and the Mx genes from some breeds were demonstrated to confer positive antiviral responses to influenza virus and vesicular stomatitis virus. On the basis of the comparison among the antiviral activities associated with many Mx variations, a specific amino acid substitution at position 631 (Ser to Asn) was considered to determine the antivirally positive or negative Mx gene. Thus, a single amino acid substitution influences the antiviral activity of Mx in domesticated chickens. |
Volume | 12(4) |
Pages | 595-601 |
Published | 2002-4-1 |
DOI | 10.1101/gr.210702 |
PMID | 11932243 |
PMC | PMC187515 |
MeSH | 3T3 Cells / virology Amino Acid Substitution / genetics Animals Antiviral Agents / genetics* Asparagine / genetics Cells, Cultured Chick Embryo / virology Chickens / genetics* GTP-Binding Proteins* Gene Expression Regulation / physiology Genetic Variation Genetic Vectors / biosynthesis Genetic Vectors / genetics Green Fluorescent Proteins Influenza A virus / growth & development Luminescent Proteins / biosynthesis Luminescent Proteins / genetics Mice Myxovirus Resistance Proteins Phylogeny Polymorphism, Genetic / genetics* Protein Biosynthesis Proteins / genetics* Proteins / physiology Serine / genetics Transfection Vesicular stomatitis Indiana virus / growth & development Virus Cultivation |
IF | 11.093 |
Times Cited | 148 |
WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | BALB/3T3 clone A31(RCB0005) |