Reference - Detail
|Author||Awazu S, Matsuoka T, Inaba K, Satoh N, Sasakura Y.|
|Title||High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis.|
The enhancer trap approach utilizing transposons yields us information about gene functions and gene expression patterns. In the ascidian Ciona intestinalis, transposon-based transgenesis and insertional mutagenesis were achieved with a Tc1/mariner transposon Minos. We report development of a novel technique for enhancer trap in C. intestinalis. This technique uses remobilization of Minos in the Ciona genome. A Minos vector for enhancer trap was constructed and a tandem array insertion of the vector was introduced into the Ciona genome to create a mutator line. Minos was remobilized in Ciona chromosomes to create new insertions by providing transposases. These transposase-introduced animals were crossed with wild-type animals. Nearly 80% of F1 families showed novel GFP expression patterns. This high-throughput enhancer trap screen will be useful to create new marker transgenic lines showing reporter gene expression in specific tissues and to identify novel patterns of gene expression.
|MeSH||Animals Animals, Genetically Modified Base Sequence Ciona intestinalis / genetics* DNA / genetics DNA Transposable Elements* Enhancer Elements, Genetic* Gene Expression Genes, Reporter Genetic Techniques Genetic Vectors Green Fluorescent Proteins / genetics In Situ Hybridization, Fluorescence Recombinant Proteins / genetics Transposases / genetics|
|WOS Category||GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY|
|C.intestinalis / (O.japonicus)||Mu[ISMiTSAdTPOG]1 E[MiTSAdTPOG]24 E[MiTSAdTPOG]22 E[MiTSAdTPOG]20 E[MiTSAdTPOG]19 E[MiTSAdTPOG]18 E[MiTSAdTPOG]17 E[MiTSAdTPOG]16 E[MiTSAdTPOG]15 E[MiTSAdTPOG]10 etc.|