RRC ID 4155
Author Joly JS, Kano S, Matsuoka T, Auger H, Hirayama K, Satoh N, Awazu S, Legendre L, Sasakura Y.
Title Culture of Ciona intestinalis in closed systems.
Journal Dev. Dyn.
Abstract Improvements in closed-system culturing methods for marine invertebrates are important prerequisites for the generalized use of transgenic lines. We discuss here the effects of several closed-system conditions on the growth and survival of the solitary ascidian, Ciona intestinalis. In Shimoda, close to the sea, a small-tank system was used to ensure that tanks and systems were reasonably equipped, water exchange was rapid, and animals separated to minimize the risk of infection. In Gif-sur-Yvette, an inland site, we tried to determine the optimal conditions to limit handling operations, and to save artificial seawater by avoiding water pollution. A mixture of at least two types of live algae was better than any single-organism diet. With these maintenance protocols, we were able to obtain several generations of Ciona intestinalis, including several transgenic lines. Because these systems make it easier to rear Ciona intestinalis in laboratories, they increase the potentialities of this model organism for research.
Volume 236(7)
Pages 1832-40
Published 2007-7
DOI 10.1002/dvdy.21124
PMID 17394236
MeSH Animals Animals, Genetically Modified Aquaculture* Ciona intestinalis* / genetics Seawater
IF 2.507
Times Cited 45
C.intestinalis / (O.japonicus) Tg[MiCiNutG]4 Tg[MiCiNutG]3 Tg[MiCiBraG]2 Tg[MiCiBraG]1 Tg[MiCiEpiIG]4 Tg[MiCiEpiIG]3 Tg[MiCiTnIG]2 Tg[MiCiTnIG]1 pSPCiEpiIG pMiCiNutG etc.