Reference - Detail
| RRC ID | 41579 |
|---|---|
| Author | Chong JM, Sakuma K, Sudo M, Osawa T, Ohara E, Uozaki H, Shibahara J, Kuroiwa K, Tominaga S, Hippo Y, Aburatani H, Funata N, Fukayama M. |
| Title | Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection. |
| Journal | J Virol |
| Abstract |
The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC. |
| Volume | 76(13) |
| Pages | 6825-31 |
| Published | 2002-7-1 |
| DOI | 10.1128/jvi.76.13.6825-6831.2002 |
| PMID | 12050395 |
| PMC | PMC136266 |
| MeSH | Adenocarcinoma / metabolism* Adenocarcinoma / virology Animals Epstein-Barr Virus Infections / metabolism* Herpesvirus 4, Human Humans Interleukin-1 / genetics Interleukin-1 / metabolism* Mice Mice, SCID Neoplasm Transplantation Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics RNA, Messenger / metabolism Stomach Neoplasms / metabolism* Stomach Neoplasms / virology Tumor Cells, Cultured |
| IF | 4.501 |
| Times Cited | 40 |
| WOS Category | VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MKN7(RCB0999) MKN45(RCB1001) MKN74(RCB1002) |