| RRC ID |
41579
|
| Author |
Chong JM, Sakuma K, Sudo M, Osawa T, Ohara E, Uozaki H, Shibahara J, Kuroiwa K, Tominaga S, Hippo Y, Aburatani H, Funata N, Fukayama M.
|
| Title |
Interleukin-1beta expression in human gastric carcinoma with Epstein-Barr virus infection.
|
| Journal |
J Virol
|
| Abstract |
The KT tumor is a transplantable strain of a human Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), established in severe combined immunodeficiency (SCID) mice, with which the cytokine expression of EBVaGC can be investigated without interference from the infiltrating lymphocytes. As a part of a high-density oligonucleotide array (GeneChip) analysis of EBVaGC, the interleukin-1beta (IL-1beta) gene was the only cytokine gene that showed markedly higher expression in the KT tumor cells than in two tumor strains of EBV-negative GC. The results were confirmed by Northern blotting, Western blotting, and enzyme-linked immunosorbent assay. Furthermore, we demonstrated a positive signal for IL-1beta mRNA in the carcinoma cells of a surgically resected EBVaGC, but not in EBV-negative GC, by in situ hybridization. In vitro, IL-1beta increased the cell growth of a GC cell line, TMK1. Thus, IL-1beta may act as an autocrine growth factor in EBVaGC.
|
| Volume |
76(13)
|
| Pages |
6825-31
|
| Published |
2002-7-1
|
| DOI |
10.1128/jvi.76.13.6825-6831.2002
|
| PMID |
12050395
|
| PMC |
PMC136266
|
| MeSH |
Adenocarcinoma / metabolism*
Adenocarcinoma / virology
Animals
Epstein-Barr Virus Infections / metabolism*
Herpesvirus 4, Human
Humans
Interleukin-1 / genetics
Interleukin-1 / metabolism*
Mice
Mice, SCID
Neoplasm Transplantation
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Stomach Neoplasms / metabolism*
Stomach Neoplasms / virology
Tumor Cells, Cultured
|
| IF |
4.501
|
| Times Cited |
40
|
|
WOS Category
|
VIROLOGY
|
| Resource |
| Human and Animal Cells |
MKN7(RCB0999)
MKN45(RCB1001)
MKN74(RCB1002) |
