| RRC ID |
41588
|
| Author |
Hinoi E, Fujimori S, Takemori A, Yoneda Y.
|
| Title |
Cell death by pyruvate deficiency in proliferative cultured calvarial osteoblasts.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Cell survival was significantly decreased in primary cultured rat calvarial osteoblasts in vitro at Day 0, 1, and 3 by replacement of the standard culture medium (alpha-modified minimum essential medium; alpha-MEM) with Dulbecco's modified eagle's medium (DMEM). Decreased cell survival was also observed following medium replacement in cultures of murine calvaria-derived osteoblastic cell line MC3T3-E1. Staining with Hoechst33342 revealed apoptotic cells with fragmented or condensed nuclei, while a fraction of the cell culture was stained with propidum iodide, indicating necrosis. Marked increases in DNA binding of both activator protein-1 and nuclear factor-kappaB were found in nuclear extracts of cells following medium replacement. The addition of either pyruvate or cysteine at each concentration found in alpha-MEM almost entirely prevented cell death associated with medium replacement at Day 3. These results suggest that pyruvate and cysteine may be essential factors for cell growth and survival in osteoblast cultures at the proliferative phase.
|
| Volume |
294(5)
|
| Pages |
1177-83
|
| Published |
2002-6-28
|
| DOI |
10.1016/S0006-291X(02)00578-8
|
| PII |
S0006-291X(02)00578-8
|
| PMID |
12074601
|
| MeSH |
Animals
Animals, Newborn
Apoptosis
Cell Division
Cell Line
Cell Survival
Cells, Cultured
Culture Media
Kinetics
Mice
NF-kappa B / metabolism
Necrosis
Osteoblasts / cytology*
Osteoblasts / drug effects
Osteoblasts / metabolism
Pyruvic Acid / pharmacology*
Rats
Rats, Wistar
Skull / cytology
Transcription Factor AP-1 / metabolism
|
| IF |
2.985
|
| Times Cited |
31
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
