RRC ID 41594
Author Rumi MA, Sato H, Ishihara S, Ortega C, Kadowaki Y, Kinoshita Y.
Title Growth inhibition of esophageal squamous carcinoma cells by peroxisome proliferator-activated receptor-gamma ligands.
Journal J Lab Clin Med
Abstract The growth of human cancer cells expressing peroxisome proliferator-activated receptor-gamma (PPAR-gamma) has been reported to be inhibited by PPAR-gamma ligands. In esophageal squamous-cell carcinoma cell lines T.T, T.Tn, and EC-GI-10, we detected expression of PPAR-gamma and investigated the effects of PPAR-gamma ligands on these cell lines in vitro with the use of troglitazone, pioglitazone, and 15d-PGJ2. Marked growth inhibition by the PPAR-gamma ligands was observed in all cases. The growth-inhibitory effect was evidenced by a dose-dependent inhibition of deoxyribonucleic acid synthesis and was associated with altered cell-cycle progression manifesting G1 arrest. Cell-cycle arrest in T.Tn cells induced by troglitazone could be correlated with an increased level of cyclin-dependent kinase inhibitor p27(Kip1), p21(Cip1/Waf1), and p18(Ink4c). Moreover, troglitazone treatment increased the expression of interleukin-1 alpha, a multifunctional cytokine implicated in antitumor immunity. These findings suggest that troglitazone and other PPAR-gamma ligands have adjuvant or neoadjuvant therapeutic potentials in esophageal cancer.
Volume 140(1)
Pages 17-26
Published 2002-7-1
DOI 10.1067/mlc.2002.125055
PII S0022214302000136
PMID 12080324
MeSH Carcinoma, Squamous Cell / pathology* Cell Cycle / drug effects Cell Division / physiology Chromans / pharmacology* DNA Replication / drug effects Esophageal Neoplasms / pathology* Gene Expression Regulation, Neoplastic* / drug effects Humans Ligands RNA, Messenger / genetics Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology* Thiazoles / pharmacology* Thiazolidinediones* Transcription Factors / genetics Transcription Factors / physiology* Transcription, Genetic* Troglitazone Tumor Cells, Cultured
Times Cited 31
Human and Animal Cells MKN-45(RCB1001) KG-1(RCB1166)