論文 - 詳細
| RRC ID | 41596 |
|---|---|
| 著者 | Kawano K, Nakamura K, Hayashi K, Nagai T, Takayama K, Maitani Y. |
| タイトル | Liver targeting liposomes containing beta-sitosterol glucoside with regard to penetration-enhancing effect on HepG2 cells. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
The aim of this study was to examine the interaction of soybean-derived sterylglucoside (SG) with the human hepatoblastoma cell line HepG2 with regard to the penetration-enhancing effect of beta-sitosterol glucoside (Sit-G) to clarify the accumulation of SG-containing liposomes (SG-liposomes) to the liver in vivo. The approach was based on measurement of the association of SG-liposomes labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil) in terms of asialoglycoprotein receptor (ASGP-R)-mediated endocytosis, affinity of Sit-G using lAsys and the association of FITC-dextran 4400 (FD-4) increased by Sit-G with the cells. The association of SG-liposomes was decreased by addition of asialofetuin, suggesting that SG-liposomes might be taken up via ASGP-R. Sit-G showed higher affinity with HepG2 cells than HeLa cells, and enhanced the association of FD-4 depending on the incubation time and Sit-G concentrations. Significant positive correlations were found between Sit-G and FD-4 association with the cells, indicating that Sit-G enhanced the drug penetration by distribution in cell membranes. The high degree of liver association of SG-liposomes in vivo might be related to recognition of glucose residues of SG by ASGP-R and to the high affinity and penetration-enhancing effect of Sit-G with hepatocytes. |
| 巻・号 | 25(6) |
| ページ | 766-70 |
| 公開日 | 2002-6-1 |
| DOI | 10.1248/bpb.25.766 |
| PMID | 12081144 |
| MeSH | Asialoglycoprotein Receptor / metabolism Asialoglycoproteins / metabolism Binding, Competitive / drug effects Carcinoma, Hepatocellular / metabolism Cell Membrane / drug effects Cell Membrane / metabolism Cell Membrane Permeability / drug effects Excipients / metabolism Fetuins Fluorescein-5-isothiocyanate Humans Liposomes / metabolism* Liver / metabolism* Liver Neoplasms / metabolism Sitosterols / metabolism* Tumor Cells, Cultured alpha-Fetoproteins / metabolism |
| IF | 1.863 |
| 引用数 | 12 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0468) |