| RRC ID |
41601
|
| 著者 |
Yu J, Leung WK, Ebert MP, Ng EK, Go MY, Wang HB, Chung SC, Malfertheiner P, Sung JJ.
|
| タイトル |
Increased expression of survivin in gastric cancer patients and in first degree relatives.
|
| ジャーナル |
Br J Cancer
|
| Abstract |
Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer.
|
| 巻・号 |
87(1)
|
| ページ |
91-7
|
| 公開日 |
2002-7-1
|
| DOI |
10.1038/sj.bjc.6600421
|
| PII |
6600421
|
| PMID |
12085263
|
| PMC |
PMC2364284
|
| MeSH |
Adult
Aged
Aged, 80 and over
Apoptosis
Blotting, Western
Chromosomal Proteins, Non-Histone / biosynthesis*
Cyclooxygenase 2
Female
Gene Expression Regulation, Neoplastic*
Genetic Predisposition to Disease*
Helicobacter Infections / complications
Humans
Immunohistochemistry
Inhibitor of Apoptosis Proteins
Isoenzymes / biosynthesis*
Male
Membrane Proteins
Microtubule-Associated Proteins*
Middle Aged
Neoplasm Proteins
Pedigree
Prostaglandin-Endoperoxide Synthases / biosynthesis*
RNA, Messenger / analysis
Stomach Neoplasms / genetics*
Stomach Neoplasms / physiopathology*
Survivin
Tumor Cells, Cultured
|
| IF |
5.791
|
| 引用数 |
46
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001)
MKN28(RCB1000) |
