論文 - 詳細
| RRC ID | 41601 |
|---|---|
| 著者 | Yu J, Leung WK, Ebert MP, Ng EK, Go MY, Wang HB, Chung SC, Malfertheiner P, Sung JJ. |
| タイトル | Increased expression of survivin in gastric cancer patients and in first degree relatives. |
| ジャーナル | Br J Cancer |
| Abstract |
Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P<0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P=0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P=0.001) and protein levels (P=0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P=0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Co-expression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. |
| 巻・号 | 87(1) |
| ページ | 91-7 |
| 公開日 | 2002-7-1 |
| DOI | 10.1038/sj.bjc.6600421 |
| PII | 6600421 |
| PMID | 12085263 |
| PMC | PMC2364284 |
| MeSH | Adult Aged Aged, 80 and over Apoptosis Blotting, Western Chromosomal Proteins, Non-Histone / biosynthesis* Cyclooxygenase 2 Female Gene Expression Regulation, Neoplastic* Genetic Predisposition to Disease* Helicobacter Infections / complications Humans Immunohistochemistry Inhibitor of Apoptosis Proteins Isoenzymes / biosynthesis* Male Membrane Proteins Microtubule-Associated Proteins* Middle Aged Neoplasm Proteins Pedigree Prostaglandin-Endoperoxide Synthases / biosynthesis* RNA, Messenger / analysis Stomach Neoplasms / genetics* Stomach Neoplasms / physiopathology* Survivin Tumor Cells, Cultured |
| IF | 5.791 |
| 引用数 | 46 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) MKN28(RCB1000) |