RRC ID |
41609
|
Author |
Kishikawa H, Miura S, Yoshida H, Hirokawa M, Nakamizo H, Higuchi H, Adachi M, Nakatsumi RC, Suzuki H, Saito H, Ishii H.
|
Title |
Transmural pressure induces IL-6 secretion by intestinal epithelial cells.
|
Journal |
Clin Exp Immunol
|
Abstract |
Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80 mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 cells at 12 h. Under control conditions, IL-6 secretion was directed to the basolateral side, but after exposure to pressure IL-6 secretion was increased in both the apical and basolateral sides. A nuclear factor kappa B (NF-kappaB) decoy reversed completely the pressure-induced increase of IL-6 secretion by IEC-18 cells. Pressure treatment enhanced IL-6 mRNA expression in IECs within 6 h. Pressure loading significantly enhanced the activation of both NF-kappaB and NF-IL-6 from 1h in the nuclear protein of IEC-18 cells as assessed by the electrophoretic mobility shift assay using FITC-conjugated specific primers. Increased phosphorylation of I-kappa B was also demonstrated in the cytosol of IEC cells within 1h by Western blot analysis. These results suggest a possible role for pressure loading in immune modulation of the intestinal mucosa by the stimulation of IL-6 release from intestinal epithelial cells.
|
Volume |
129(1)
|
Pages |
86-91
|
Published |
2002-7-1
|
DOI |
10.1046/j.1365-2249.2002.01895.x
|
PII |
1895
|
PMID |
12100026
|
PMC |
PMC1906408
|
MeSH |
Animals
CCAAT-Enhancer-Binding Protein-beta / metabolism
Cell Line / metabolism
Cell Polarity
DNA-Binding Proteins / metabolism
Electrophoretic Mobility Shift Assay
Epithelial Cells / metabolism
Gene Expression Regulation / physiology*
I-kappa B Proteins*
Interleukin-6 / biosynthesis
Interleukin-6 / genetics
Interleukin-6 / metabolism*
Intestinal Mucosa / metabolism*
NF-KappaB Inhibitor alpha
NF-kappa B / antagonists & inhibitors
NF-kappa B / metabolism
Phosphorylation
Pressure*
Protein Processing, Post-Translational
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Rats
Stress, Mechanical
Time Factors
Transcription, Genetic
|
IF |
3.532
|
Times Cited |
11
|
WOS Category
|
IMMUNOLOGY
|
Resource |
Human and Animal Cells |
7-TD-1(RCB1190) |