RRC ID |
41616
|
Author |
Ichino N, Yamada K, Nishii K, Sawada H, Nagatsu T, Ishiguro H.
|
Title |
Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells.
|
Journal |
J Neural Transm (Vienna)
|
Abstract |
The influence of nicotine on the expression of egr-1 and nur77 genes by nicotine treatment and withdrawal was assessed using PC12 cells. Nicotine treatment significantly increased the amount of mRNA for egr-1 and nur77 genes at 0.5 h post-nicotine treatment in the PC12 cells. In addition, nicotine withdrawal also elevated transcriptional levels of egr-1 and nur77 genes in Northern blot analyses. Nicotine treatment (200 microM) was also found to significantly increase expressional levels of Egr-1 and Nur77 proteins at 0.5 h post-nicotine treatment. In contrast, Egr-1 and Nur77 protein levels were dramatically decreased by nicotine withdrawal. These results suggest that expressional levels of Egr-1 and Nur77 proteins in neural cells may affect the transcriptional activity of late-response genes after nicotine withdrawal.
|
Volume |
109(7-8)
|
Pages |
1015-22
|
Published |
2002-7-1
|
DOI |
10.1007/s007020200084
|
PMID |
12111438
|
MeSH |
Animals
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Early Growth Response Protein 1
Immediate-Early Proteins*
Nicotine / adverse effects*
Nicotine / pharmacology
Nuclear Receptor Subfamily 4, Group A, Member 1
PC12 Cells / drug effects
PC12 Cells / physiology*
RNA, Messenger / metabolism
Rats
Receptors, Cytoplasmic and Nuclear
Receptors, Steroid
Substance Withdrawal Syndrome / genetics*
Substance Withdrawal Syndrome / metabolism
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcription, Genetic / drug effects*
|
IF |
3.505
|
Times Cited |
12
|
WOS Category
|
CLINICAL NEUROLOGY
NEUROSCIENCES
|
Resource |
Human and Animal Cells |
|