RRC ID 4163
Author Shibuya K, Yamada T, Suzuki T, Shimizu K, Ichimura K.
Title InPSR26, a putative membrane protein, regulates programmed cell death during petal senescence in Japanese morning glory.
Journal Plant Physiol.
Abstract The onset and progression of petal senescence, which is a type of programmed cell death (PCD), are highly regulated. Genes showing changes in expression during petal senescence in Japanese morning glory (Ipomoea nil) were isolated and examined to elucidate their function in PCD. We show here that a putative membrane protein, InPSR26, regulates progression of PCD during petal senescence in Japanese morning glory. InPSR26 is dominantly expressed in petal limbs and its transcript level increases prior to visible senescence symptoms. Transgenic plants with reduced InPSR26 expression (PSR26r lines) showed accelerated petal wilting, with PCD symptoms including cell collapse, ion and anthocyanin leakage, and DNA degradation accelerated in petals compared to wild-type plants. Transcript levels of autophagy- and PCD-related genes (InATG4, InATG8, InVPE, and InBI-1) were reduced in the petals of PSR26r plants. Autophagy visualized by monodansylcadaverine staining confirmed that autophagy is induced in senescing petal cells of wild-type plants and that the percentage of cells containing monodansylcadaverine-stained structures, most likely autophagosomes, was significantly lower in the petals of PSR26r plants, indicating reduced autophagic activity in the PSR26r plants. These results suggest that InPSR26 acts to delay the progression of PCD during petal senescence, possibly through regulation of the autophagic process. Our data also suggest that autophagy delays PCD in petal senescence.
Volume 149(2)
Pages 816-24
Published 2009-2
DOI 10.1104/pp.108.127415
PII pp.108.127415
PMID 19036837
PMC PMC2633835
MeSH Anthocyanins / metabolism Apoptosis / physiology* Cellular Senescence / physiology* Flowers / genetics Flowers / growth & development* Ipomoea nil / cytology Ipomoea nil / genetics* Ipomoea nil / growth & development* Membrane Proteins / genetics* Plant Proteins / genetics* Plants, Genetically Modified / genetics RNA, Messenger / genetics RNA, Plant / genetics Up-Regulation
IF 6.305
Times Cited 16
Morning Glory