Reference - Detail
| RRC ID | 41637 |
|---|---|
| Author | Kanazawa T, Kono T, Watanabe M, Matsushima-Hibiya Y, Nakano T, Koyama K, Tanaka N, Sugimura T, Wakabayashi K. |
| Title | Bcl-2 blocks apoptosis caused by pierisin-1, a guanine-specific ADP-ribosylating toxin from the cabbage butterfly. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Pierisin-1, a 98-kDa protein that induces apoptosis in mammalian cell lines, is capable of being incorporated into cells where it ADP-ribosylates guanine residues in DNA. To investigate the apoptotic pathway induced by this unique protein, the bcl-2 gene was transfected into HeLa cells. Cy2-fluorescent pierisin-1 was incorporated into the resultant cells expressing Bcl-2 protein and ADP-ribosylated dG was detected to almost the same extent as in parent cells. However, bcl-2-transfected HeLa cells did not display apoptotic morphological changes, PARP cleavage, and DNA fragmentation, indicating acquisition of resistance. In parent HeLa cells, activation of caspase-9 and release of cytochrome c were observed after 8h treatment with 0.5ng/ml pierisin-1. Caspase substrate assays revealed further cleavage of Ac-DEVD-pNA, Ac-VDVAD-pNA, and Ac-VEID-pNA, suggesting activation of caspase-2, -3, and -6 in pierisin-1-treated HeLa cells. The caspase-3 inhibitor, Ac-DEVD-CHO, was also found to inhibit apoptosis. In contrast, this caspase activation was not observed in bcl-2-transfected HeLa cells. Our results thus indicate that pierisin-1-induced apoptosis is mediated primarily via a mitochondrial pathway involving Bcl-2 and caspases. |
| Volume | 296(1) |
| Pages | 20-5 |
| Published | 2002-8-9 |
| DOI | 10.1016/s0006-291x(02)00815-x |
| PII | S0006291X0200815X |
| PMID | 12147221 |
| MeSH | ADP Ribose Transferases Adenosine Diphosphate / metabolism* Animals Apoptosis / drug effects Apoptosis / genetics* Butterflies Caspases / metabolism Cytochrome c Group / metabolism Enzyme Activation Genes, bcl-2* HeLa Cells Humans Insect Proteins / pharmacology* |
| IF | 2.985 |
| Times Cited | 19 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |