Reference - Detail
| RRC ID | 41643 |
|---|---|
| Author | Nagase M, Shiota T, Tsushima A, Murshedul Alam M, Fukuoka S, Yoshizawa T, Sakato N. |
| Title | Molecular mechanism of satratoxin-induced apoptosis in HL-60 cells: activation of caspase-8 and caspase-9 is involved in activation of caspase-3. |
| Journal | Immunol Lett |
| Abstract |
Satratoxins have been recognized as potential immunomodulatory agents in outbreaks of building-related illness. Here we report that satratoxin G-treated human leukemia HL-60 cells underwent apoptosis through the action of caspase-3 which was activated by both caspase-8 and caspase-9. Western blot analysis of caspase-3 in the satratoxin G-treated cells apparently indicated the appearance of a catalytically active fragment of 17 kDa. Increased caspase-3 activity was also detected by using a fluorogenic substrate, DEVD-AMC. Next, exposure to satratoxin G led to cleavage of PARP from its native 116 kDa form to a 85 kDa product. Moreover, DFF-45/ICAD were cleaved into a 12.5 kDa fragment via satratoxin G treatment. Enzymic assay on IETD-AMC revealed that caspase-8 is strongly activated by exposure to satratoxin G while T-2 toxin (T-2) could not activate caspase-8 at an early stage of apoptosis. Furthermore, satratoxin G caused a release of cytochrome c from mitochondria into the cytosol and increased the activity of caspase-9 against LEHD-AMC. These findings indicate that satratoxin G-induced apoptosis involves activation of caspase-3 and DFF-40/CAD through both activation of caspase-8 and cytosolic accumulation of cytochrome c along with activation of caspase-9. |
| Volume | 84(1) |
| Pages | 23-7 |
| Published | 2002-10-21 |
| DOI | 10.1016/s0165-2478(02)00127-x |
| PII | S016524780200127X |
| PMID | 12161280 |
| MeSH | Apoptosis / drug effects* Caspase 3 Caspase 8 Caspase 9 Caspases / metabolism* Coumarins Cytochrome c Group / metabolism Deoxyribonucleases / metabolism Enzyme Activation / drug effects Fluorescent Dyes HL-60 Cells Humans Immunosuppressive Agents / toxicity Oligopeptides Poly(ADP-ribose) Polymerases / metabolism Poly-ADP-Ribose Binding Proteins Sick Building Syndrome / etiology Stachybotrys / pathogenicity Trichothecenes / toxicity* |
| IF | 3.276 |
| Times Cited | 25 |
| WOS Category | IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HL60(RCB0041) |