RRC ID 41703
Author Hascilowicz T, Murai N, Matsufuji S, Murakami Y.
Title Regulation of ornithine decarboxylase by antizymes and antizyme inhibitor in zebrafish (Danio rerio).
Journal Biochim. Biophys. Acta
Abstract Mammalian polyamine synthesis is regulated by a unique feedback mechanism. When cellular polyamine levels increase, antizyme, an ornithine decarboxylase (ODC) inhibitory protein, is induced by polyamine-dependent translational frameshifting. Antizyme not only inhibits ODC, a key enzyme in polyamine synthesis, it also targets the enzyme degradation by the 26S proteasome. Furthermore, it suppresses cellular uptake of polyamines. Previously, we isolated two zebrafish antizymes with different expressions and activities. This suggested that a common feedback mechanism of polyamine metabolism might operate in mammals and zebrafish (Danio rerio). In the present study, cDNAs of zebrafish ODC and antizyme inhibitor, another regulatory protein that inhibits antizyme action, were cloned. The presence of ODC and antizyme inhibitor mRNAs was confirmed by Northern blotting in embryos and adult fish, as well as in a zebrafish-derived cell line (BRF41). The activity of the ODC cDNA expression product was inhibited by short and long zebrafish antizymes, and recombinant zebrafish antizyme inhibitor reversed this inhibition. In the BRF41 cells, the ODC half-life was considerably longer than that of mammalian ODC but shorter than that of Schizosaccharomyces pombe. Spermidine elicited a rapid decay of ODC activity and ODC protein in a protein synthesis-dependent manner.
Volume 1578(1-3)
Pages 21-8
Published 2002-10-11
DOI 10.1016/s0167-4781(02)00476-1
PII S0167478102004761
PMID 12393184
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA, Complementary / biosynthesis DNA, Complementary / isolation & purification Molecular Sequence Data Ornithine Decarboxylase / genetics Ornithine Decarboxylase / metabolism* Ornithine Decarboxylase Inhibitors Polyamines / metabolism Protein Isoforms / genetics Proteins / genetics Proteins / metabolism* RNA, Messenger / biosynthesis Recombinant Proteins / metabolism Spermidine / pharmacology Zebrafish / embryology Zebrafish / genetics Zebrafish / metabolism*
IF 3.79
Times Cited 15
Human and Animal Cells