論文 - 詳細
| RRC ID | 41704 |
|---|---|
| 著者 | Takahashi S, Sato H, Kubota Y, Utsumi H, Bedford JS, Okayasu R. |
| タイトル | Inhibition of DNA-double strand break repair by antimony compounds. |
| ジャーナル | Toxicology |
| Abstract |
DNA double strand breaks (DSBs), induced by gamma-irradiation in Chinese hamster ovary cells, were used to examine whether antimony compounds affect the repair of DNA damage. The cells were first incubated with antimony trichloride or antimony potassium tartrate (both Sb(III)) for 2 h, and then irradiated with gamma-rays at a dose of 40 Gy. The DNA DSB was quantified with pulsed field gel electrophoresis immediately after irradiation (non-repair group) as well as at 30 min post-irradiation (repair group). The degree of repair inhibition was determined by the differences in the amount of DNA DSB between non-repair and repair groups. Both antimony compounds inhibited repair of DNA DSB in a dose dependent manner. In trichloride, 0.2 mM antimony significantly inhibited the rejoining of DSB, while 0.4 mM was necessary in potassium antimony tartrate. The mean lethal doses, D(0), for the treatment with antimony trichloride and antimony potassium tartrate, were approximately 0.21 and 0.12 mM, respectively. This indicates that the repair inhibition by antimony trichloride occurred in the dose range near D(0), but the antimony potassium tartrate inhibited the repair at doses where most cells lost their proliferating ability. This is the first report to indicate that antimony compounds may inhibit the repair of radiation-induced DNA DSB. |
| 巻・号 | 180(3) |
| ページ | 249-56 |
| 公開日 | 2002-12-2 |
| DOI | 10.1016/s0300-483x(02)00401-8 |
| PII | S0300483X02004018 |
| PMID | 12393294 |
| MeSH | Animals Antimony / pharmacology* Antimony Potassium Tartrate / pharmacology CHO Cells Cell Adhesion / drug effects Cell Survival / drug effects Colony-Forming Units Assay Cricetinae DNA / drug effects* DNA / radiation effects DNA Damage / drug effects* DNA Damage / radiation effects DNA Repair / drug effects* DNA Repair / radiation effects Gamma Rays |
| IF | 4.099 |
| 引用数 | 31 |
| WOS 分野 | PHARMACOLOGY & PHARMACY TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |