| RRC ID |
41708
|
| 著者 |
Sakakura C, Hagiwara A, Nakanishi M, Shimomura K, Takagi T, Yasuoka R, Fujita Y, Abe T, Ichikawa Y, Takahashi S, Ishikawa T, Nishizuka I, Morita T, Shimada H, Okazaki Y, Hayashizaki Y, Yamagishi H.
|
| タイトル |
Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites.
|
| ジャーナル |
Br J Cancer
|
| Abstract |
Advanced gastric cancer is often accompanied by metastasis to the peritoneum, resulting in a high mortality rate. Mechanisms involved in gastric cancer metastasis have not been fully clarified because metastasis involves multiple steps and requires a combination of altered expressions of many different genes. Thus, independent analysis of any single gene would be insufficient to understand all of the aspects of gastric cancer peritoneal dissemination. In this study, we performed a global analysis of the differential gene expression of a gastric cancer cell line established from a primary main tumour (SNU-1) and of other cell lines established from the metastasis to the peritoneal cavity (SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB). The application of a high-density cDNA microarray method made it possible to analyse the expression of approximately 21 168 genes. Our examinations of SNU-5, SNU-16, SNU-620, KATO-III and GT3TKB showed that 24 genes were up-regulated and 17 genes down-regulated besides expression sequence tags. The analysis revealed the following altered expression such as: (a) up-regulation of CD44 (cell adhesion), keratins 7, 8, and 14 (epitherial marker), aldehyde dehydrogenase (drug metabolism), CD9 and IP3 receptor type3 (signal transduction); (b) down-regulation of IL2 receptor gamma, IL4-Stat (immune response), p27 (cell cycle) and integrin beta4 (adhesion) in gastric cancer cells from malignant ascites. We then analysed eight gastric cancer cell lines with Northern blot and observed preferential up-regulation and down-regulation of these selected genes in cells prone to peritoneal dissemination. Reverse transcriptase-polymerase chain reaction confirmed that several genes selected by DNA microarray were also overexpressed in clinical samples of malignant ascites. It is therefore considered that these genes may be related to the peritoneal dissemination of gastric cancers. The results of this global gene expression analysis of gastric cancer cells with peritoneal dissemination, promise to provide a new insight into the study of human gastric cancer peritoneal dissemination.
|
| 巻・号 |
87(10)
|
| ページ |
1153-61
|
| 公開日 |
2002-11-4
|
| DOI |
10.1038/sj.bjc.6600580
|
| PII |
6600580
|
| PMID |
12402156
|
| PMC |
PMC2376186
|
| MeSH |
Animals
Apoptosis
Cell Adhesion
Cell Cycle
Cell Movement
Female
Gene Expression Profiling*
Humans
Mice
Mice, Nude
Neoplasm Transplantation
Peritoneal Neoplasms / genetics*
Peritoneal Neoplasms / secondary
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics
Stomach Neoplasms / genetics*
Stomach Neoplasms / immunology
Stomach Neoplasms / pathology
Tumor Cells, Cultured
|
| IF |
5.791
|
| 引用数 |
68
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
GT3TKB(RCB0885)
MKN7(RCB0999) |
