| RRC ID |
41711
|
| 著者 |
Saijo M, Suzutani T, De Clercq E, Niikura M, Maeda A, Morikawa S, Kurane I.
|
| タイトル |
Genotypic and phenotypic characterization of the thymidine kinase of ACV-resistant HSV-1 derived from an acyclovir-sensitive herpes simplex virus type 1 strain.
|
| ジャーナル |
Antiviral Res
|
| Abstract |
Twenty-four strains of acyclovir (ACV)-resistant (ACV(r)) herpes simplex virus type 1 (HSV-1) were generated from the HSV-1 TAS strain by exposure to ACV, and the genotype and phenotype of the thymidine kinase (TK) from these mutants were analyzed. The TK polypeptide of the ACV(r) HSV-1 strains was examined by Western blot using an anti-HSV-1 TK rabbit serum. The sensitivity of each strain to ACV, foscarnet and cidofovir (CDV) was also determined. A single guanine (G) insertion or a single cytosine (C) deletion was detected in 12 of the 24 ACV(r) strains at the G or C homopolymer stretches within the TK gene. Genotypic analysis predicted that two thirds of the ACV(r) HSV-1 strains expressed truncated TK polypeptides, while one third expressed viral TK polypeptide with a single amino acid substitution at various sites. Western blot abnormalities in the viral TK polypeptides were identified in 21 ACV(r) strains. There was an inverse correlation between the susceptibility of the HSV-1 mutant strains to ACV and that to CDV. Nucleotide sequencing of the TK gene and Western blot analysis of the viral TK polypeptides are considered to be one of the methods for predicting virus sensitivity to ACV and CDV.
|
| 巻・号 |
56(3)
|
| ページ |
253-62
|
| 公開日 |
2002-12-1
|
| DOI |
10.1016/s0166-3542(02)00131-6
|
| PII |
S0166354202001316
|
| PMID |
12406508
|
| MeSH |
Acyclovir / pharmacology*
Amino Acid Sequence
Animals
Antiviral Agents / pharmacology*
Base Sequence
Blotting, Western
Chlorocebus aethiops
Cidofovir
Cytosine / analogs & derivatives*
Cytosine / pharmacology
Drug Resistance, Viral / genetics
Herpesvirus 1, Human / drug effects*
Herpesvirus 1, Human / enzymology*
Herpesvirus 1, Human / genetics
Herpesvirus 1, Human / metabolism
Molecular Sequence Data
Mutation / genetics
Organophosphonates*
Organophosphorus Compounds / pharmacology
Sequence Analysis, DNA
Thymidine Kinase / genetics*
Thymidine Kinase / metabolism
Vero Cells
Viral Plaque Assay
|
| IF |
4.101
|
| 引用数 |
30
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
VIROLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
