論文 - 詳細
| RRC ID | 41720 |
|---|---|
| 著者 | Tomita T, Nagase T, Ohga E, Yamaguchi Y, Yoshizumi M, Ouchi Y. |
| タイトル | Molecular mechanisms underlying human beta-defensin-2 gene expression in a human airway cell line (LC2/ad). |
| ジャーナル | Respirology |
| Abstract |
OBJECTIVE:Recently, human beta-defensin-2 (hBD-2), an inducible defensin, has been reported to be involved in innate immunity and host defence. To examine the exact roles of hBD-2 in the respiratory system, we examined the molecular mechanisms of hBD-2 gene expression in vitro. METHODOLOGY:Using a human airway cell line (LC-2/ad), lipopolysaccharide (LPS)-induced gene expression of hBD-2 was studied in the absence or the presence of (i) dexamethasone, (ii) inhibition of NF-kappaB and AP-1, (iii) intracellular calcium chelator, and (iv) cyclooxygenase (COX) inhibitors. RESULTS:Lipopolysaccharide-induced gene expression of hBD-2 was down-regulated by (i) dexamethasone, (ii) inhibition of NF-kappaB and AP-1, and (iii) intracellular calcium chelator. However, COX inhibitors had no effect on LPS-induced mRNA expression of hBD-2. CONCLUSION:These findings suggest that glucocorticoids (GC), but not COX inhibitors, reduce hBD-2 gene expression, while NF-kappaB, AP-1 and intracellular calcium are essential for hBD-2 expression. Glucocorticoid-induced down-regulation of hBD-2 might be involved in the GC-induced suppression of respiratory host defence associated with hBD-2. |
| 巻・号 | 7(4) |
| ページ | 305-10 |
| 公開日 | 2002-12-1 |
| DOI | 10.1046/j.1440-1843.2002.00415.x |
| PII | 415 |
| PMID | 12421237 |
| MeSH | Blotting, Northern Calcium / physiology Cell Line Cyclooxygenase Inhibitors / pharmacology Dexamethasone / pharmacology Down-Regulation Gene Expression Glucocorticoids / pharmacology Humans Lipopolysaccharides / pharmacology NF-kappa B / physiology Respiratory System / cytology Respiratory System / immunology* Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / physiology beta-Defensins / genetics* |
| IF | 4.88 |
| 引用数 | 24 |
| WOS 分野 | RESPIRATORY SYSTEM |
| リソース情報 | |
| ヒト・動物細胞 | LC-2/ad(RCB0440) |