RRC ID 41729
Author Sugiura T, Nakane S, Kishimoto S, Waku K, Yoshioka Y, Tokumura A.
Title Lysophosphatidic acid, a growth factor-like lipid, in the saliva.
Journal J. Lipid Res.
Abstract Lysophosphatidic acid is a multifunctional phospholipid mediator and elicits a variety of biological responses in vitro and in vivo. Evidence is accumulating that lysophosphatidic acid plays important physiological roles in diverse mammalian tissues and cells. In the present study, we first examined whether lysophosphatidic acid is present in human saliva. We found that a significant amount of lysophosphatidic acid is present in the saliva (0.785 nmol/ml). The predominant fatty acyl moiety of lysophosphatidic acid was 18:1n-9 + n-7 followed by 18:0 and 16:0. A small amount of lysoplasmanic acid, an alkyl ether-linked analog of lysophosphatidic acid, was also detected in the saliva (0.104 nmol/ml). We found that physiologically relevant concentrations of lysophosphatidic acid induced accelerated growth of cells of mouth, pharynx, and esophagus origin in vitro. Lysophosphatidic acid also induced rapid increases in the intracellular free Ca2+ concentrations in these cells. We obtained evidence that lysophosphatidic acid receptor mRNAs are actually present in these cells. These results strongly suggest that lysophosphatidic acid is involved in wound healing in the upper digestive organs such as the mouth, pharynx, and esophagus.
Volume 43(12)
Pages 2049-55
Published 2002-12
DOI 10.1194/jlr.m200242-jlr200
PMID 12454265
MeSH Calcium / metabolism Epithelium / growth & development Epithelium / metabolism Esophagus / growth & development Esophagus / metabolism Humans Lysophospholipids / chemistry Lysophospholipids / metabolism* Mass Spectrometry RNA, Messenger / metabolism Receptors, Cell Surface / biosynthesis Receptors, Cell Surface / genetics Receptors, G-Protein-Coupled* Receptors, Lysophosphatidic Acid Saliva / chemistry Saliva / metabolism* Tumor Cells, Cultured
IF 4.505
Times Cited 71
Human and Animal Cells