論文 - 詳細
| RRC ID | 41731 |
|---|---|
| 著者 | Ajuh P, Chusainow J, Ryder U, Lamond AI. |
| タイトル | A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing. |
| ジャーナル | EMBO J |
| Abstract |
Human factor C1 (HCF-1) is needed for the expression of herpes simplex virus 1 (HSV-1) immediate-early genes in infected mammalian cells. Here, we provide evidence that HCF-1 is required for spliceosome assembly and splicing in mammalian nuclear extracts. HCF-1 interacts with complexes containing splicing snRNPs in uninfected mammalian cells and is a stable component of the spliceosome complex. We show that a missense mutation in HCF-1 in the BHK21 hamster cell line tsBN67, at the non-permissive temperature, inhibits the protein's interaction with U1 and U5 splicing snRNPs, causes inefficient spliceosome assembly and inhibits splicing. Transient expression of wild-type HCF-1 in tsBN67 cells restores splicing at the non-permissive temperature. The inhibition of splicing in tsBN67 cells correlates with the temperature-sensitive cell cycle arrest phenotype, suggesting that HCF-1-dependent splicing events may be required for cell cycle progression. |
| 巻・号 | 21(23) |
| ページ | 6590-602 |
| 公開日 | 2002-12-2 |
| DOI | 10.1093/emboj/cdf652 |
| PMID | 12456665 |
| PMC | PMC136956 |
| MeSH | Cell Nucleus / metabolism Herpes Simplex / metabolism* Host Cell Factor C1 Humans In Vitro Techniques Mutation Proteins / genetics Proteins / metabolism* RNA Splicing* RNA, Messenger / metabolism* Ribonucleoproteins, Small Nuclear / metabolism Spliceosomes / metabolism Transcription Factors* |
| IF | 9.889 |
| 引用数 | 19 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BHK-21C13-2P(RCB0420) tsBN67(RCB1271) |