| RRC ID |
41731
|
| 著者 |
Ajuh P, Chusainow J, Ryder U, Lamond AI.
|
| タイトル |
A novel function for human factor C1 (HCF-1), a host protein required for herpes simplex virus infection, in pre-mRNA splicing.
|
| ジャーナル |
EMBO J
|
| Abstract |
Human factor C1 (HCF-1) is needed for the expression of herpes simplex virus 1 (HSV-1) immediate-early genes in infected mammalian cells. Here, we provide evidence that HCF-1 is required for spliceosome assembly and splicing in mammalian nuclear extracts. HCF-1 interacts with complexes containing splicing snRNPs in uninfected mammalian cells and is a stable component of the spliceosome complex. We show that a missense mutation in HCF-1 in the BHK21 hamster cell line tsBN67, at the non-permissive temperature, inhibits the protein's interaction with U1 and U5 splicing snRNPs, causes inefficient spliceosome assembly and inhibits splicing. Transient expression of wild-type HCF-1 in tsBN67 cells restores splicing at the non-permissive temperature. The inhibition of splicing in tsBN67 cells correlates with the temperature-sensitive cell cycle arrest phenotype, suggesting that HCF-1-dependent splicing events may be required for cell cycle progression.
|
| 巻・号 |
21(23)
|
| ページ |
6590-602
|
| 公開日 |
2002-12-2
|
| DOI |
10.1093/emboj/cdf652
|
| PMID |
12456665
|
| PMC |
PMC136956
|
| MeSH |
Cell Nucleus / metabolism
Herpes Simplex / metabolism*
Host Cell Factor C1
Humans
In Vitro Techniques
Mutation
Proteins / genetics
Proteins / metabolism*
RNA Splicing*
RNA, Messenger / metabolism*
Ribonucleoproteins, Small Nuclear / metabolism
Spliceosomes / metabolism
Transcription Factors*
|
| IF |
9.889
|
| 引用数 |
19
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
