| RRC ID |
41734
|
| Author |
Ikenoue T, Ijichi H, Kato N, Kanai F, Masaki T, Rengifo W, Okamoto M, Matsumura M, Kawabe T, Shiratori Y, Omata M.
|
| Title |
Analysis of the beta-catenin/T cell factor signaling pathway in 36 gastrointestinal and liver cancer cells.
|
| Journal |
Jpn J Cancer Res
|
| Abstract |
We investigated the frequency and mechanism of beta-catenin/T cell factor (Tcf) signaling activation in a panel of 36 human gastrointestinal and liver cancer cell lines. Reporter assay and electrophoretic mobility shift assay revealed that the beta-catenin/Tcf signaling was upregulated in 12 of 12 (100%) colorectal, 5 of 8 (68%) gastric, 2 of 7 (29%) hepatic, and none of 9 pancreatic cancer cell lines. The activation of the pathway was mainly due to the mutation of adenomatous polyposis coli (APC) or beta-catenin, and Tcf-4 was highly expressed in these cell lines with upregulated signaling. Nuclear beta-catenin was observed not only in the signaling-activated cell lines, but also in 14 of 25 (56%) primary gastric cancers, 15 of 20 (75%) colon cancers, 5 of 19 (26%) hepatocellular carcinomas, and none of 13 pancreatic cancers. The presence of signaling-upregulated gastric cancer cell lines with intact APC and beta-catenin suggests the involvement of other mechanisms than mutations of APC or beta-catenin.
|
| Volume |
93(11)
|
| Pages |
1213-20
|
| Published |
2002-11-1
|
| DOI |
10.1111/j.1349-7006.2002.tb01226.x
|
| PMID |
12460462
|
| PMC |
PMC5926899
|
| MeSH |
Carcinoma, Hepatocellular / etiology*
Colorectal Neoplasms / etiology*
Cytoskeletal Proteins / analysis
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / physiology*
DNA-Binding Proteins / analysis
DNA-Binding Proteins / physiology*
Genes, APC
Humans
Immunohistochemistry
Liver Neoplasms / etiology*
Lymphoid Enhancer-Binding Factor 1
Stomach Neoplasms / etiology*
Trans-Activators / analysis
Trans-Activators / genetics
Trans-Activators / physiology*
Transcription Factors / analysis
Transcription Factors / physiology*
Tumor Cells, Cultured
Up-Regulation
beta Catenin
|
| Times Cited |
43
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
|
