論文 - 詳細
| RRC ID | 41760 |
|---|---|
| 著者 | Shigeta K, Ootaki K, Tatemoto H, Nakanishi T, Inada A, Muto N. |
| タイトル | Potentiation of nerve growth factor-induced neurite outgrowth in PC12 cells by a Coptidis Rhizoma extract and protoberberine alkaloids. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells. |
| 巻・号 | 66(11) |
| ページ | 2491-4 |
| 公開日 | 2002-11-1 |
| DOI | 10.1271/bbb.66.2491 |
| PMID | 12506995 |
| MeSH | Animals Berberine / analogs & derivatives* Berberine / pharmacology Berberine Alkaloids / isolation & purification Berberine Alkaloids / pharmacology* Cholinesterase Inhibitors / pharmacology Chromatography, High Pressure Liquid / methods Coptis chinensis Dose-Response Relationship, Drug Drugs, Chinese Herbal / chemistry* Drugs, Chinese Herbal / pharmacology Enzyme Activation / physiology Nerve Growth Factors / pharmacology* Neurites / drug effects* Neurites / physiology PC12 Cells Rats |
| IF | 1.516 |
| 引用数 | 39 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-12(RCB0009) |