| RRC ID |
41760
|
| 著者 |
Shigeta K, Ootaki K, Tatemoto H, Nakanishi T, Inada A, Muto N.
|
| タイトル |
Potentiation of nerve growth factor-induced neurite outgrowth in PC12 cells by a Coptidis Rhizoma extract and protoberberine alkaloids.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells.
|
| 巻・号 |
66(11)
|
| ページ |
2491-4
|
| 公開日 |
2002-11-1
|
| DOI |
10.1271/bbb.66.2491
|
| PMID |
12506995
|
| MeSH |
Animals
Berberine / analogs & derivatives*
Berberine / pharmacology
Berberine Alkaloids / isolation & purification
Berberine Alkaloids / pharmacology*
Cholinesterase Inhibitors / pharmacology
Chromatography, High Pressure Liquid / methods
Coptis chinensis
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / chemistry*
Drugs, Chinese Herbal / pharmacology
Enzyme Activation / physiology
Nerve Growth Factors / pharmacology*
Neurites / drug effects*
Neurites / physiology
PC12 Cells
Rats
|
| IF |
1.516
|
| 引用数 |
39
|
|
WOS 分野
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
