Reference - Detail
| RRC ID | 41769 |
|---|---|
| Author | Chang KS, Ohashi K, Onuma M. |
| Title | Suppression of transcription activity of the MEQ protein of oncogenic Marek's disease virus serotype 1 (MDV1) by L-MEQ of non-oncogenic MDV1. |
| Journal | J Vet Med Sci |
| Abstract |
Meq is one of the candidate oncogenes in the MDV1 genome. We previously reported a difference in the meq open reading frame (ORF) between oncogenic and non-oncogenic MDV1: L-meq, in which a 180-bp sequence is inserted into the meq ORF, is detected in non-oncogenic MDV1. To study the functions of a gene product of L-meq (L-MEQ), transactivation by L-MEQ was analyzed by dual luciferase assay using a reporter gene under the control of long (-1--873 bp) and short (-1 - -355 bp) meq promoter (LMP and SMP, respectively). LMP showed higher promoter function than SMP. L-MEQ transactivated the expression of the reporter gene, but less than MEQ did. In the presence of SMP or the cytomegalovirus immediate-early promoter, the same or slightly higher transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq. However, in the presence of LMP, lower transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq, suggesting that L-MEQ can be a transrepressor. Replication of a vvMDV1 was enhanced in the cells with meq. Interestingly, however, replication of vvMDV1 was suppressed in the cells with L-meq or with both L-meq and meq, compared to untransfected cells. Thus, L-MEQ could suppress replication of vvMDV1 displaying the meq gene in coinfected cells. |
| Volume | 64(12) |
| Pages | 1091-5 |
| Published | 2002-12-1 |
| DOI | 10.1292/jvms.64.1091 |
| PMID | 12520099 |
| MeSH | Animals Antiviral Agents / genetics Antiviral Agents / metabolism Cells, Cultured Gene Expression Regulation, Viral* Herpesvirus 2, Gallid / genetics* Herpesvirus 2, Gallid / metabolism* Humans Mice Oncogene Proteins, Viral / genetics* Oncogene Proteins, Viral / metabolism* Promoter Regions, Genetic / genetics Transcription, Genetic* Transcriptional Activation Tumor Cells, Cultured Virus Replication |
| IF | 1.049 |
| Times Cited | 23 |
| WOS Category | VETERINARY SCIENCES |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 3T3 Swiss Albino(RCB1642) Hep G2 |