RRC ID 4177
Author McMiller TL, Sims D, Lee T, Williams T, Johnson CM.
Title Molecular characterization of the Caenorhabditis elegans REF-1 family member, hlh-29/hlh-28.
Journal Biochim. Biophys. Acta
Abstract Members of the Caenorhabditis elegans REF-1 family of bHLH proteins are atypical in that each protein contains two bHLH domains. In this study we describe a functional and molecular characterization of the REF-1 family members, hlh-29/hlh-28. 5'-RACE results confirm the presence of two bHLH domain coding regions in a single transcript and quantitative PCR (qPCR) shows that hlh-29/hlh-28 mRNA is detected in wild-type animals throughout development. A promoter fusion of hlh-29 to the green fluorescent protein shows post-embryonic reporter activity in cells of the vulva, the somatic gonad, the intestine and in neuronal cells of the head and tail. Loss of hlh-29/hlh-28 function via RNA interference (RNAi) results in multiple phenotypes including late embryonic lethality, yolk protein accumulation, everted vulva, bordering behavior, and alter chemosensory responses.
Volume 1769(1)
Pages 5-19
Published 2007-1
DOI 10.1016/j.bbaexp.2006.12.001
PII S0167-4781(06)00208-9
PMID 17258327
MeSH Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors / chemistry Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / pharmacology Basic Helix-Loop-Helix Transcription Factors / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / pharmacology Caenorhabditis elegans Proteins / physiology* Embryo, Nonmammalian Embryonic Development / genetics Female Gene Expression Regulation, Developmental Gonads / embryology Gonads / metabolism Molecular Sequence Data Neurons / metabolism RNA Interference Tissue Distribution Vulva / embryology Vulva / metabolism
IF 3.438
Times Cited 5
C.elegans tm458 tm284