| RRC ID |
41786
|
| Author |
Takeda K, Yokoyama S, Yasumoto Ki, Saito H, Udono T, Takahashi K, Shibahara S.
|
| Title |
OTX2 regulates expression of DOPAchrome tautomerase in human retinal pigment epithelium.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Otx2 is a member of homeodomain-containing transcription factors and is essential for eye morphogenesis in mice. Here we show the expression of OTX2, the human counterpart of Otx2, in cell lines of retinal pigment epithelium (RPE) and in Y79 retinoblastoma cells that exhibit the property of presumptive RPE. These RPE cells express DOPAchrome tautomerase (DCT) that is an enzyme involved in melanin biosynthesis. DCT may contribute to the homeostasis of RPE by detoxifying DOPA-derived metabolites. OTX2 binds to the DCT gene promoter in vivo, as judged by chromatin immunoprecipitation assays. Furthermore, repression of endogenous OTX2 expression in Y79 cells by an anti-sense OTX2 oligonucleotide resulted in the decrease of DCT protein contents. Transient expression assays revealed that OTX2 activated the DCT gene promoter through the OTX-2-binding site in an RPE-specific manner. Therefore, OTX2 may regulate RPE-specific target genes, such as DCT, thereby maintaining the homeostasis of RPE.
|
| Volume |
300(4)
|
| Pages |
908-14
|
| Published |
2003-1-24
|
| DOI |
10.1016/s0006-291x(02)02934-0
|
| PII |
S0006291X02029340
|
| PMID |
12559959
|
| MeSH |
Adolescent
Animals
Cell Line
Child
Gene Expression Regulation, Enzymologic*
Genes, Reporter
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Homeostasis
Humans
Intramolecular Oxidoreductases / genetics
Intramolecular Oxidoreductases / metabolism*
Male
Mice
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Oligonucleotides, Antisense / metabolism
Otx Transcription Factors
Pigment Epithelium of Eye / cytology
Pigment Epithelium of Eye / enzymology
Pigment Epithelium of Eye / physiology*
Promoter Regions, Genetic
Trans-Activators / genetics
Trans-Activators / metabolism*
Tumor Cells, Cultured
|
| IF |
2.985
|
| Times Cited |
33
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HMV-II(RCB0777)
Y79(RCB1645) |
