RRC ID 41792
著者 Okamura H, Morimoto H, Fujita M, Nasu F, Sasakia E, Haneji T.
タイトル Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid.
ジャーナル Oral Oncol
Abstract We examined the expression of early growth response-1 (Egr-1) gene in human oral squamous carcinoma cell lines SCCKN and SCC-25 cells and human osteoblastic cell lines Saos-2 and MG63 cells treated with okadaic acid, a potent inhibitor of protein phosphatases type 1 and type 2A. Western blot analysis revealed that Egr-1 was strongly expressed in SCCKN cells and that okadaic acid decreased the expression of Egr-1 protein in these cells. However, Egr-1 was expressed at lower levels in SCC-25, Saos-2, and MG63 cells and transiently increased with the okadaic acid treatment. Suppression of Egr-1 protein expression in okadaic acid-treated SCCKN cells stemmed from the suppression of the Egr-1 mRNA level, as determined by the RT-RCR method. Formaldehyde-fixed and alcohol-permeabilized cultured SCCKN cells were reacted with the anti-Egr-1 antibody using immunohistochemical methods. Intense fluorescence was observed in the nuclei of the control SCCKN cells interacted with anti-Egr-1 antibody. However, only a weak reaction was observed in the nuclei in SCCKN cells treated with okadaic acid. A gel mobility shift assay showed that treatment of SCCKN cells with okadaic acid suppressed Egr-1 binding to the DIG-labeled Egr-1 consensus oligonucleotide probe. The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level.
巻・号 38(8)
ページ 779-84
公開日 2002-12-1
DOI 10.1016/s1368-8375(02)00039-8
PII S1368-8375(02)00039-8
PMID 12570057
MeSH Carcinogens / therapeutic use* Carcinoma, Squamous Cell / drug therapy* Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Down-Regulation Early Growth Response Protein 1 Electrophoresis, Agar Gel / methods Gene Expression Regulation Humans Immediate-Early Proteins* Immunohistochemistry / methods Mouth Neoplasms / drug therapy* Mouth Neoplasms / genetics Mouth Neoplasms / metabolism Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Okadaic Acid / therapeutic use* Reverse Transcriptase Polymerase Chain Reaction / methods Suppression, Genetic Transcription Factors / genetics Transcription Factors / metabolism* Tumor Cells, Cultured
IF 3.979
引用数 7
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE ONCOLOGY
リソース情報
ヒト・動物細胞 SCCKN(RCB0441)