| RRC ID |
41805
|
| 著者 |
Goto T, Kato N, Yoshida H, Otsuka M, Moriyama M, Shiratori Y, Koike K, Matsumura M, Omata M.
|
| タイトル |
Synergistic activation of the serum response element-dependent pathway by hepatitis B virus x protein and large-isoform hepatitis delta antigen.
|
| ジャーナル |
J Infect Dis
|
| Abstract |
Hepatitis delta virus (HDV) is a naturally occurring satellite of hepatitis B virus (HBV). There are few studies of the effects of the combination of HBV and HDV proteins (HDV antigens [HDAgs]) on intracellular signaling pathways. To understand the influence of HBV and HDV coinfection on hepatocytes, we investigated the effect of HBV proteins and HDAgs on the serum response element (SRE)-dependent pathway. Reporter assays revealed that only HBV X protein (HBx), alone or with the large isoform of HDAg (LHDAg), synergistically activated the SRE-dependent pathway. The effect of HBx and LHDAg on Elk1 or serum response factor (SRF) was examined, because both proteins bind to the SRE. HBx activated the transcriptional ability of Elk1, whereas LHDAg activated the transcriptional ability of SRF. Thus, HBx and LHDAg synergistically activated the SRE-dependent pathway. These results may help us understand clinical phenomena in patients coinfected with HBV and HDV.
|
| 巻・号 |
187(5)
|
| ページ |
820-8
|
| 公開日 |
2003-3-1
|
| DOI |
10.1086/368389
|
| PII |
JID020549
|
| PMID |
12599056
|
| MeSH |
Animals
COS Cells
DNA-Binding Proteins*
HeLa Cells
Hepatitis delta Antigens / genetics
Hepatitis delta Antigens / metabolism*
Humans
Protein Isoforms
Proto-Oncogene Proteins / metabolism
Serum Response Element / genetics*
Serum Response Factor / metabolism
Signal Transduction*
Trans-Activators / genetics
Trans-Activators / metabolism*
Transcription Factors*
Transcription, Genetic
Transcriptional Activation*
Transfection
Tumor Cells, Cultured
Viral Regulatory and Accessory Proteins
ets-Domain Protein Elk-1
|
| IF |
5.022
|
| 引用数 |
14
|
|
WOS 分野
|
INFECTIOUS DISEASES
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
COS-7(RCB0539)
HuH-7(RCB1366) |
