Reference - Detail
| RRC ID | 41821 |
|---|---|
| Author | Matsuguchi T, Takagi A, Matsuzaki T, Nagaoka M, Ishikawa K, Yokokura T, Yoshikai Y. |
| Title | Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through Toll-like receptor 2. |
| Journal | Clin Diagn Lab Immunol |
| Abstract |
Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-alpha) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-kappaB activation and TNF-alpha production in RAW264.7, a mouse macrophage cell line, the activity was found to be as follows: protoplast > cell wall > polysaccharide-peptidoglycan complex. Both crude extracts and purified lipoteichoic acids (LTAs) from two Lactobacillus strains, L. casei and L. fermentum, significantly induced TNF-alpha secretion from RAW264.7 cells and splenocytes of C57BL/6, C3H/HeN, and C3H/HeJ mice but not from splenocytes of C57BL/6 TLR2(-/-) mice. Lactobacillus LTA induced activation of c-Jun N-terminal kinase activation in RAW264.7 cells. Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-kappaB was activated in response to Lactobacillus LTA. Taken together, these data suggest that LTAs from lactobacilli elicit proinflammatory activities through TLR2. |
| Volume | 10(2) |
| Pages | 259-66 |
| Published | 2003-3-1 |
| DOI | 10.1128/cdli.10.2.259-266.2003 |
| PMID | 12626452 |
| PMC | PMC150522 |
| MeSH | Animals Cell Line Humans Lactobacillus / immunology* Lipopolysaccharide Receptors / genetics Lipopolysaccharides / immunology* Macrophages / cytology Macrophages / immunology* Macrophages / microbiology Membrane Glycoproteins / genetics Membrane Glycoproteins / immunology* Membrane Glycoproteins / metabolism Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Mutant Strains NF-kappa B / metabolism Protoplasts / immunology Receptors, Cell Surface / genetics Receptors, Cell Surface / immunology* Receptors, Cell Surface / metabolism Spleen / cytology Teichoic Acids / immunology* Toll-Like Receptor 2 Toll-Like Receptors Transfection Tumor Necrosis Factor-alpha / immunology* Tumor Necrosis Factor-alpha / metabolism |
| Times Cited | 171 |
| WOS Category | INFECTIOUS DISEASES MICROBIOLOGY IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 20 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) 293T(RCB2202) |