論文 - 詳細
| RRC ID | 41830 |
|---|---|
| 著者 | Imachi H, Murao K, Cao W, Tada S, Taminato T, Wong NC, Takahara J, Ishida T. |
| タイトル | Expression of human scavenger receptor B1 on and in human platelets. |
| ジャーナル | Arterioscler Thromb Vasc Biol |
| Abstract |
OBJECTIVE:The abundance of HDL particles correlates inversely with the incidence of coronary heart disease. The human scavenger receptor B1 (hSR-B1/CLA-1) is a receptor for HDL. Expression of hSR-B1/CLA-1 mRNA and protein in human platelets was determined using reverse transcriptase-polymerase chain reaction and Western blot, respectively. Presence of the protein on the surface of platelets was shown using flow cytometry. METHODS AND RESULTS:Immunochemical staining for hSR-B1/CLA-1 showed that it was expressed in megakaryocytes, the platelet precursors of human bone marrow. These findings prompted us to ask whether hSR-B1/CLA-1 was differentially expressed on platelets obtained from patients with atherosclerotic disease compared with those in control subjects. Our findings showed that abundance of hSR-B1/CLA-1 was significantly reduced on the surface of platelets from patients with atherosclerotic disease. The reduced levels of hSR-B1/CLA-1 were associated with increased cholesterol ester content in platelets from patients with atherosclerotic disease compared with control subjects. A negative correlation existed between hSR-B1/CLA-1 expression and platelet aggregation. In summary, our studies show that the HDL receptor hSR-B1/CLA-1 is expressed in platelets and their precursor, the megakaryocyte. The levels of hSR-B1/CLA-1 expression correlate inversely with cholesterol ester content and platelet aggregation. CONCLUSIONS:These findings suggest that determining the level of hSR-B1/CLA-1 expression on the platelets may be a useful clinical marker for atherosclerotic diseases. |
| 巻・号 | 23(5) |
| ページ | 898-904 |
| 公開日 | 2003-5-1 |
| DOI | 10.1161/01.ATV.0000067429.46333.7B |
| PII | 01.ATV.0000067429.46333.7B |
| PMID | 12649086 |
| MeSH | Adult Aged Arteriosclerosis / blood* Biomarkers Blood Platelets / chemistry* CD36 Antigens / blood* CD36 Antigens / genetics Cell Membrane / chemistry Cells, Cultured Cholesterol / metabolism Cholesterol Esters / blood Female Humans Male Megakaryocytes / chemistry Membrane Proteins* Middle Aged Platelet Aggregation RNA, Messenger / blood Receptors, Immunologic* Receptors, Lipoprotein* Receptors, Scavenger Scavenger Receptors, Class B Transfection |
| IF | 6.604 |
| 引用数 | 60 |
| WOS 分野 | HEMATOLOGY PERIPHERAL VASCULAR DISEASE |
| リソース情報 | |
| ヒト・動物細胞 | |