論文 - 詳細
| RRC ID | 41843 |
|---|---|
| 著者 | Inada Y, Nakane T, Chiba S. |
| タイトル | Relationship between ligand binding and YIPP motif in the C-terminal region of human AT1 receptor. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
The YIPP (tyrosine-isoleucine-proline-proline, amino acids 319-322) motif within the C-terminal part of the human AT(1) receptor is associated with angiotensin II (AII)-induced activation of the Jak-STAT pathway and phospholipase Cgamma1 phosphorylation. We report here that mutations of the YIPP motif strongly affect ligand-binding to the receptor. We analysed AT(1) receptors of the wild type (WT) and 11 mutants with a FLAG-epitope-tag within their C-terminal portion. Mutations of the "P-P" amino acid sequence of this motif decreased both AII binding and the AII-induced intracellular Ca(2+) transients. Mutant and WT receptors were expressed equally in the cell membrane and were localized within the plasma membrane. These results suggest that the "P-P" amino acid sequence within the YIPP motif is important for AII binding to the AT(1) receptor. |
| 巻・号 | 1640(1) |
| ページ | 33-41 |
| 公開日 | 2003-4-7 |
| DOI | 10.1016/s0167-4889(02)00400-7 |
| PII | S0167488902004007 |
| PMID | 12676352 |
| MeSH | Amino Acid Sequence Angiotensin II / metabolism Animals Blotting, Western COS Cells Calcium / metabolism Humans Iodine Radioisotopes Ligands Molecular Sequence Data Mutation Phospholipase C gamma Phosphorylation Proline / chemistry Radioligand Assay Receptor, Angiotensin, Type 1 Receptors, Angiotensin / chemistry Receptors, Angiotensin / genetics Receptors, Angiotensin / metabolism* Type C Phospholipases / metabolism |
| IF | 3.411 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |