論文 - 詳細
| RRC ID | 41853 |
|---|---|
| 著者 | Kitamura H, Matsushita Y, Iwanaga T, Mori K, Kanehira K, Fujikura D, Morimatsu M, Saito M. |
| タイトル | Bacterial lipopolysaccharide-induced expression of the IkappaB protein MAIL in B-lymphocytes and macrophages. |
| ジャーナル | Arch Histol Cytol |
| Abstract |
Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL), a recently cloned nuclear IkappaB protein induced by lipopolysaccharide (LPS) stimulation in lymphoid organs, is involved in the regulation of inflammatory responses. The present in situ hybridization and immunohistochemical analyses revealed the distinct expression of the MAIL mRNA and protein in B-lymphocytes of the white pulp of the spleen and cortical lymphoid follicles of lymph nodes in LPS-injected mice. MAIL signals were also localized in F4/80-positive macrophages in these organs. LPS clearly induced MAIL expression in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. MAIL was also induced by inflammatory cytokines such as interleukin-1 and -6, and tumor necrosis factor in cultured cells. Northern blot, Western blot, and in situ hybridization analyses showed that the major expression product of the Mail gene was a long splicing variant (MAIL-L) rather than a short one, both in lymphoid organs and cultured cells. These results collectively indicate that LPS induces MAIL-L predominantly in B-lymphocytes and macrophages. |
| 巻・号 | 66(1) |
| ページ | 53-62 |
| 公開日 | 2003-3-1 |
| DOI | 10.1679/aohc.66.53 |
| PMID | 12703554 |
| MeSH | Adaptor Proteins, Signal Transducing Animals B-Lymphocytes / cytology B-Lymphocytes / drug effects B-Lymphocytes / physiology* Blotting, Western Cell Line Gene Expression / drug effects Gene Expression / immunology Immunohistochemistry In Situ Hybridization Lipopolysaccharides / pharmacology* Lymphoid Tissue / cytology Macrophages, Peritoneal / cytology Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / physiology* Male Mice Mice, Inbred C57BL Nuclear Proteins / genetics* Nuclear Proteins / metabolism RNA, Messenger / analysis T-Lymphocytes / cytology T-Lymphocytes / drug effects T-Lymphocytes / physiology |
| 引用数 | 13 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CTLL-2(RCB0637) 3T3 Swiss Albino(RCB1642) |