| RRC ID |
41853
|
| Author |
Kitamura H, Matsushita Y, Iwanaga T, Mori K, Kanehira K, Fujikura D, Morimatsu M, Saito M.
|
| Title |
Bacterial lipopolysaccharide-induced expression of the IkappaB protein MAIL in B-lymphocytes and macrophages.
|
| Journal |
Arch Histol Cytol
|
| Abstract |
Molecule possessing ankyrin-repeats induced by lipopolysaccharide (MAIL), a recently cloned nuclear IkappaB protein induced by lipopolysaccharide (LPS) stimulation in lymphoid organs, is involved in the regulation of inflammatory responses. The present in situ hybridization and immunohistochemical analyses revealed the distinct expression of the MAIL mRNA and protein in B-lymphocytes of the white pulp of the spleen and cortical lymphoid follicles of lymph nodes in LPS-injected mice. MAIL signals were also localized in F4/80-positive macrophages in these organs. LPS clearly induced MAIL expression in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. MAIL was also induced by inflammatory cytokines such as interleukin-1 and -6, and tumor necrosis factor in cultured cells. Northern blot, Western blot, and in situ hybridization analyses showed that the major expression product of the Mail gene was a long splicing variant (MAIL-L) rather than a short one, both in lymphoid organs and cultured cells. These results collectively indicate that LPS induces MAIL-L predominantly in B-lymphocytes and macrophages.
|
| Volume |
66(1)
|
| Pages |
53-62
|
| Published |
2003-3-1
|
| DOI |
10.1679/aohc.66.53
|
| PMID |
12703554
|
| MeSH |
Adaptor Proteins, Signal Transducing
Animals
B-Lymphocytes / cytology
B-Lymphocytes / drug effects
B-Lymphocytes / physiology*
Blotting, Western
Cell Line
Gene Expression / drug effects
Gene Expression / immunology
Immunohistochemistry
In Situ Hybridization
Lipopolysaccharides / pharmacology*
Lymphoid Tissue / cytology
Macrophages, Peritoneal / cytology
Macrophages, Peritoneal / drug effects
Macrophages, Peritoneal / physiology*
Male
Mice
Mice, Inbred C57BL
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
RNA, Messenger / analysis
T-Lymphocytes / cytology
T-Lymphocytes / drug effects
T-Lymphocytes / physiology
|
| Times Cited |
13
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
CTLL-2(RCB0637)
3T3 Swiss Albino(RCB1642) |
